CD34、CK34βE12在子宫颈病变中表达的意义

Clinical Significance of CD34 and CK34βE12 Expressions in Lesions of the Cervix

目的探讨CD34、CK34βE12在宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)中表达的意义。方法应用免疫组织化S-P法检测CD34、CK34βE12蛋白在120例慢性宫颈炎和CIN中的表达。结果在慢性宫颈炎中CD34的阳性表达率为70%,CK34βE12为86%;在CINⅠ中CD34的阳性率表达为71%,CK34βE12为81%;在CINⅡ中CD34的阳性表达率为77%,CK34βE12为66%;在CINⅢ中CD34的阳性表达率为97%,CK34βE12为100%。CD34、CK34βE12阳性表达比例有随宫颈CIN级别增高而升高的趋势(P<0.05)。2者表达与CIN级别显著相关,但2者表达之间差异无统计学意义(P>0.05)。CD34在宫颈炎和低级别的CIN病变中的阳性表达主要位于基质内的血管内皮细胞,而在高级别的CIN病变中其表达弥散,几乎所有的基质内纤维细胞都有着色,其在宫颈癌的病例中着色又变弱甚至缺如;CK34βE12的表达位于上皮内,在鳞状上皮化生处其与腺上皮有明显的界限,在高级别的CIN中表达弥散而强烈,在宫颈癌中其表达微弱甚至呈阴性。结论CD34、CK34βE12的表达与宫颈病变的程度有关,但2者的阳性表达无明显相关。CD34、CK34βE12可能与宫颈病变的发生、进展有关,可用于辅助判断CIN的级别,但不能判断宫颈癌的类型。

Objective To study the CD34 and CK34βE12 expressions in the squamous intraepithelial lesions of the cervix and their clinical significance. Methods The S-P immunohistochemical method to detect CD34 and CK34βE12 expressions was applied to in 120 samples. Results The positive rate of CD34 and CK34βE12 in the inflammatory lesions were 70% and 86% , respectively; and in CIN I the positive rate of CD34 was 71% , and the positive rate of CK34βE12 was 81% ;in CIN 11 the positive rate of CD34 was 77% , the positive rate of CK34βE12 was 66% ; in CIN m the positive rate of CD34 was 97%, while that of CK34βE12 could reach 100%. In addition, the expression pattern of the two proteins were related to the grade of cervical lesions （ P 〈 0.05 ）. Conclusion The expressions of CD34 and CK34βE12 were correlated with CIN grading and these the two protein expressions may provide certain clinical significance in the diagnosis of CINs in the cervix, but not the canncer type.