湖南省苗族健康人群外周血CD3+、CD4+、CD8+T淋巴细胞正常参考值研究

Normal reference values of peripheral blood CD3+,CD4+ and CD8+ T lymphocytes in healthy Miao ethnics in Hunan Province

目的了解并建立湖南省苗族健康人群外周血CD3＋、CD4＋、CD8＋T淋巴细胞正常参考值。方法应用FACSCalibur流式细胞仪检测10-19岁、20-29岁、30-39岁、40-49岁、50-59岁5个年龄组共360名健康苗族人的CD3＋、CD4＋、CD8＋T淋巴细胞数值。结果10-59岁组苗族人群外周血CD3＋、CD4＋、CD8＋T淋巴细胞绝对数和CD4＋／CD8＋比值分别为（1426．71±462．92）个／μL、（787．54±258．36）个／μL、（589．86±242．91）个／μL和1．46±0．50；10～19岁年龄组的CD3＋、CD8＋T淋巴细胞计数均明显高于30岁以上年龄组（F分别为10．80、8．51，均P〈0．05），CD4＋T淋巴细胞计数明显高于50-59岁组（F=7．03，P（0．05）；不同性别间上述指标无差异（t分别为0．98、0．80、0．88、0．16，均P〉0．05）。结论建立不同民族的CD3＋、CD4＋、CD8＋T淋巴细胞正常参考值有助于临床诊断。

Objective To understand and establish the normal reference values of peripheral blood CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphocytes in healthy Miao ethnics in Hunan Province. Methods Three hundred and sixty healthy Miao ethnics were divided into 5 age groups （ 10 - 19, 20 - 29, 30 -39,40 - 49, 50 - 59）, their peripheral blood CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphocytes were detected with FACSCalibur flowcytometry. Results The mean absolute values of peripheral blood CD3 ＋ , CD4 ＋ , CD8 ＋ T lyrnphocytes and CD4 ＋/CD8 ＋ in healthy Miao ethnics in 10-59 age groups were （1 426. 71 ± 462. 92） cells/μL, （787. 54 ±258. 36） cells/μL, （589. 86 ± 242. 91） cells/ μL and 1.46 ± 0. 50 respectively, the mean absolute values of peripheral blood CD3 ＋ and CD8 ＋ T lymphocytes in 10-19 age group were significantly higher than those in 30-59 age group （F = 10. 80,8. 51, respectively, all P〈 0. 05）, CD4 ＋ T lymphocytes value was obviously higher than that in 50- 59 age group （F = 7. 03, P〈0. 05）; There was on significant difference in above values between different genders （t = 0. 98, 0. 80, 0. 88, 0. 16 respectively, all P〉0. 05）. Conclusion The establishment of normal reference values of peripheral blood CD3 ＋ , CD4 ＋ and CD8 ＋ T lymphocytes for healthy Miao ethnics will help to clinical diagnosis.