摘要
Scallop Chlamys farreri was exposed to different concentrations of benzo(a)pyrene (BaP) (0.5 μg/L, 1.0 μg/L, 10.0 μg/L and 50.0 μg/L) for 30 days in seawater. The 7-ethoxyresorufin O-deethylase (EROD) activity was significantly induced, and increased with the increasing BaP concentration. The glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) activities increased in short time at low concentration of BaP, and was significantly depressed at high concentrations. Scallop gill was more sensitive to BaP than the digestive gland, and the digestive gland was the main tissue to deal with oxyradicals. The contents of malondialdehyde (MDA) increased with the exposure time and there was a positive correlation (concentration-effect) between the MDA content and the concentration of BaP. The biomarkers determined in this experiment had important roles in detoxification, and showed great potential as biomarkers for oxidative stress. Controlled laboratory experiments designed to simulate field exposure scenarios are particularly useful in ascertaining biomarkers suitable for use with complex contaminant mixtures in the marine environment.
Scallop Chlamys farreri was exposed to different concentrations of benzo（a）pyrene （BaP） （0.5μg/L, 1.0μg/L, 10.0μg/L and 50.0μg/L） for 30 days in seawater. The 7-ethoxyresorufin O-deethylase （EROD） activity was significantly induced, and increased with the increasing BaP concentration. The glutathione-S-transferase （GST）, superoxide dismutase （SOD）, catalase （CAT）, Glutathione peroxidase （GPx） activities increased in short time at low concentration of BaP, and was significantly depressed at high concentrations. Scallop gill was more sensitive to BaP than the digestive gland, and the digestive gland was the main tissue to deal with oxyradicals. The contents of malondialdehyde （MDA） increased with the exposure time and there was a positive correlation （concentration-effect） between the MDA content and the concentration of BaP. The biomarkers determined in this experiment had important roles in detoxification, and showed great potential as biomarkers for oxidative stress. Controlled laboratory experiments designed to simulate field exposure scenarios are particularly useful in ascertaining biomarkers suitable for use with complex contaminant mixtures in the marine environment.
基金
Supported by the Technology Development Program of Shandong (No. 2008GG1005010)
the Program of Introducing Talents of Discipline of Universities (111 Project, No. B08049)
