摘要
H295R细胞系能表达类固醇激素合成过程中涉及的所有酶类,生成肾上腺皮质合成的所有类固醇激素,且与正常的人肾上腺细胞对毒物的反应水平有很好的相关性,能从基因表达、酶活力和激素三个层面体外筛选环境类固醇激素干扰物和研究其作用机制,已成为体外筛选环境类固醇激素干扰物最具潜力的工具。笔者就H295R细胞系在环境类固醇激素干扰物筛选中的应用及存在的问题进行了综述。
H295R cells can express all the key enzymes involved in the synthesis of steroid hormones,and have the ability to produce the steroid hormones tound in the aduh human adrenal cortex. Good correlation of response to toxicity has been reported between H295R cell line and normal adult human adrenal cells. H295R cell line can be used to evaluate effects of chemicals on gene expression, enzymatic activities and hormone production, and to explore the mode of action of chemicals on steroidgenesis. H295R cell line has been suggested as a potentially useful tool for screening environmental steroid hormone disruptors in vitro. The application of H295R cell line for screening environmental steroid hormone disruptors in vitro and its current issues are reviewed in this article.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第4期374-375,共2页
Journal of Environment and Health
基金
国家重点基础研究发展计划项目(2007CB109106)
关键词
环境污染
内分泌干扰物
体外筛选
H295R细胞
肾上腺皮质激素干扰物
Environmental pollution
Endocrine disruptor
In vitro screening
H295R cell line
Adrenocortical steroid hormone disruptor
