琼脂糖凝胶直接杂交快速鉴定低拷贝数转基因

A Rapid Method of Identifying Low Copy Number Transgene Using Agarose Gel Hybirdization.

采用常规的ＰＣＲ方法检测低拷贝数转基因有一定困难．提出一种使用琼脂糖凝胶直接杂交的方法，结果明确、简单可行，是转基因动物中低拷贝数转基因鉴定的一种较好方法．

There are a lot of difficulties using PCR method to identify low copys of exogenous gene in transgenic animal. A method of agarose gel direct hybirdization was established. A fast and easy method to identify low copy transgenes in transgenic animal is provided.