EBV-BHRF1基因阳性与阴性细胞株对3种诱导凋亡因素抵抗性差异的研究

DIFFERENCE BETWEEN EBVBHRF1 GENE POSITIVE AND NEGATIVE CELL LINES AGAINST 3 KINDS OF APOPTOSIS INDUCING FACTORS

目的观察ＥＢＶ┐ＢＨＲＦ１基因阳性（ＳＵＮＥ┐１、Ｒａｊｉ和Ｂ９５┐８）与阴性（Ｋ５６２、ＹＡＣ）细胞株对３种诱导细胞凋亡（ａｐｏｐ┐ｔｏｓｉｓ）因素（无血清培养４８小时，４３℃作用１０分钟或２×１０－６ｍｏｌ／Ｌ地塞米松作用２４小时）的抵抗力差异。方法ＰＣＲ扩增ＥＢＶ┐ＢＨＲＦ１基因片段诱导后经电镜及电泳ＤＮＡ检测细胞凋亡。结果分别从Ｂ９５┐８、Ｒａｊｉ及人低分化鼻咽癌细胞株（ＳＵＮＥ┐１）ＤＮＡ中扩增出ＥＢ病毒的ＢＨＲＦ１全基因片段，而Ｋ５６２、ＹＡＣ细胞株则不含该基因片段。分别经上述３种方法处理后，前３种细胞株的形态及ＤＮＡ电泳行为未发生明显改变，而后２种细胞株则出现典型的细胞凋亡特征，即细胞核凝缩，细胞膜及细胞器基本保持完整；ＤＮＡ电泳出现梯形（Ｌａｄｄｅｒ）条带。

bjective To observe the difference between EBVBHRF1 gene positive and negative cell lines against 3 kinds of apoptosis inducing factors. Methods These cell lines were cultured in ①the absence of fetal calf serum for 48 h ②43℃ for 10 min,and ③2×10-6 mol/L dexamethasone for 24 h.Polymerase chain reaction (PCR) and apoptosis inducing and detecting methods were used. Results EBVBHRF1 gene fragment was amplified from B958、Raji and SUNE1 cell lines with PCR technique,while K562 and YAC cell lines were negative.The results against 3 kinds of apoptosis inducing factors from electron microscopy and agarose gel electrophoresis indicated that apoptosis appeared in BHRF1 genenegative cell lines,while BHRF1 genepositive cell lines were normal. Conclusion EBVBHRF1 gene is one of the important factors in suppressing its host cell's apoptosis。