mPEG-PLGA-mPEG系列纳米粒的毒性与其结构的关系

Preliminary investigation on the cytotoxicity-composition relationship of serials of novel mPEG-PLGA-mPEG nanoparticles

目的研究丙交酯/乙交酯/聚乙二醇(LA/GA/PEG)共聚物(mPEG-PLGA-mPEG)系列材料所制备的空白纳米粒的细胞毒性,并考察毒性与材料结构的关系。方法采用MTT法测定与空白纳米粒培养48 h后的正常人肝细胞Chang的细胞存活率,采用Origin软件分析mPEG-PLGA-mPEG材料中的PEG分子量,并考察PEG的含量和LA/GA比例对毒性的交互作用。结果PEG的分子量和含量对纳米粒的毒性影响较明显,LA/GA比例对其影响较小。结论控制合成mPEG-PLGA-mPEG的材料中所使用PEG的分子量和含量,可降低mPEG-PLGA-mPEG所制备纳米粒的毒性。

OBJECTIVE To investigate the cytotoxicity and compositon - cytotoxicity relationship of serials of novel monomethoxy （ polyethylene glycol） - poly （ d, 1 - lactic - co - glycolic acid ） - monomethoxy （ polyethylene glycol） （ mPEG - PLGA - mPEG） blank nanoparticles. METHODS MTT assay was adopted to measure the relative growth rate （RGR） of Chang liver cells after incubation with blank nanoparticles for 48 hours. And the Origin software was applied to analyze the relationships between cytotoxicity and PEG molecular weight as well as PEG content and LA/GA ratio. RESULTS The molecular weight and content of PEG had remarkable in- fluence on the cytotoxicity of nanoparticles, while the LA/GA ratio had little influence on it. CONCLUSION By controlling the PEG molecular weight and content in the synthesis of mPEG - PLGA - mPEG, the cytotoxicity of blank nanoparticles would be reduced. And by this means the innoxious and safe nanoparticle materials for injection would be developed.