5-脂氧合酶在大鼠急性坏死性胰腺炎组织中的表达

Expression of 5-lipoxygenase in rat pancreatic tissue during acute necrotizing pancreatitis

目的:研究5-脂氧合酶(5-lipoxygenase,5-LOX)在急性坏死性胰腺炎(ANP)病程中的表达.方法:将SD大鼠54只随机分为3组:假手术组、ANP组和齐留通组.经十二指肠行胆胰管逆行加压注射50g/L牛磺胆酸钠,诱导大鼠ANP模型.各组大鼠分别于6、12、24h处死.用RT-PCR、免疫组化和Westernblot免疫印迹法检测胰腺组织5-LOXmRNA及相应蛋白的表达.ELISA方法检测血清LTB4水平.应用全自动生化分析仪检测各组大鼠血清淀粉酶水平.结果:与ANP组相比较,齐留通组各个时点5-LOX蛋白及mRNA表达均显著降低,差异具有统计学意义(1.333%±0.516%vs2.667%±0.516%,3.000%±0.632%vs4.500%±0.548%,3.833%±0.753%vs5.833%±0.408%;0.285%±0.005%vs0.366%±0.004%,0.608%±0.005%vs0.949%±0.013%,0.297%±0.002%vs0.400%±0.006%,均P<0.05).假手术组血清淀粉酶、LTB4水平很低,ANP组和齐留通组水平都明显升高,且齐留通组每个时点都较ANP组水平降低,差异有统计学意义(血清淀粉酶:1967.50±41.21IU/Lvs2123.50±49.11IU/L,3242.33±87.76IU/Lvs3531.17±74.14IU/L,2286.83±93.91IU/Lvs2903.33±90.90IU/L,均P<0.05).结论:急性胰腺炎时,5-LOX表达明显增加,使用齐留通后,5-LOX表达明显减少.

AIM： To investigate the expression of 5-1ipoxygenase in rat pancreatic tissue during acute necrotizing pancreatitis. METHODS： Fifty four SD rats were randomized into 3 groups： sham operation group, acute necrotizing pancreatitis group （ANP group） and zileuton treatment group. The model of ANP was induced by retrograde injection of 5% sodium taurocholate into biliopancreatic duct. At 6, 12, 24 h, the animals were sacrificed. Immunohistochemistry and Western blot were used to detect the expression of 5qipoxygenase of pancreas. The mRNA expression of 5-1ipoxygennase in the pancreas was determined using RT- PCR. The serum level of LTB4 was analyzed by enzyme-linked immunosorbent assay （ELISA）. The serum level of amylase was detected. RESULTS： The levels of 5-LOX and 5-LOX mRNA were significantly lower at each time point in zileuton group than in ANP group （1.333%0.516% vs 2.667% ± 0.516%, 3.000% ± 0.632% vs 4.500% ± 0.548%, 3.833% ± 0.753% vs 5.833% ± 0.408%; 0.285% ± 0.005% vs 0.366% ± 0.004% 0.608% ± 0.005% vs 0.949% ±_ 0.013%, 0.297% ± 0.002% vs 0.400% ± 0.006%, all P 〈 0.05）. Compared with sham operation group, ANP group and zileuton group had significantly elevated serum levels of amylase and LTB4. However, serum levels of amylase and LTB4 were significantly lower in zileuton group than in ANP group （amylase： 1967.50 ± 41.21 IU/L vs 2123.50 ± 49.11 IU/L, 3242.33 ± 87.76 IU/L vs 3531.17 ± 74.14 IU/L, 2286.83 ± 93.91 IU/L vs 2903.33 ± 90.90 IU/L, all P 〈 0.05）. CONCLUSION： The expression of 5-LOX is significantly higher during acute necrotizing pancreatitis.