Study on the Fermentation Conditions of a Mucopolysaccharide-producing Bacterium LV-1

一株粘性多糖产生菌LV-1的发酵条件研究(英文)

bacterium LV-1 which isolated from soil sample were studied bacterium was isolated by serial dilution method, the effects of carbon source, nitrogen source, the initial pH and temperature on producing polysaccharide by it were discussed to confirm the optimum fermentation conditions. [ Result] The physicochemical properties showed that the polysaccharide was water-soluble, but insoluble in organic solvents including ethanol, butanol, and chloroform. It was neutral polysaccharide with negative charge and without reducing terminal. The pH of its solution was pH =7.5. There were no protein, fructose, uronic acid, sulphate and starch-like structure included in po/ysacchadde molecules. The optimum fermentation conditions for po/ysaccharide produc- tion were 3% mannitol as carbon source, 0.25% yeast extract as nitrogen source, culture temperature 28 ~（3 and pH =7.5. [Coadu^en] The re- search could provide basis for development and utilization of LV-1 and industrialized production of mucopolysaccharide.

1材料与方法1．1粘性多糖产生菌的分离与培养1．1．1菌株分离。将2g土样加入100ml含有3g异麦芽酮糖和0．15g酵母浸粉的培养基中，28℃摇床（150r／min）培养2d，然后将菌悬液用生理盐水梯度稀释法涂平板，于28℃培养箱内培养过夜。