老年人外周血树突状细胞的体外培养及功能研究

Study on the culture and function of dendritic cells derived from the very elderly in vitro

目的探讨≥80岁老年人外周血单核细胞体外诱导培养生成树突状细胞（dendritic cells,DCs）的情况及其功能特点。方法取30例≥80岁老年人及30例60~70岁老年人外周血,用淋巴细胞分离液离心获得单个核细胞,贴壁培养2 h后获得单核细胞,用含有rhGM-CSF和rhIL-4的培养基培养1周,观察2组DCs的生长、活力、存活时间、同种T淋巴细胞的刺激反应,并进行比较。结果≥80岁组DCs培养过程中细胞逐渐死亡,培养至第7天大部分细胞死亡,获得的DCs数量较少,其中有8例未能培养出DCs;较60~70岁组对同种T淋巴细胞的刺激能力也显著下降,而60~70岁组培养细胞的活力保持较好,至第7天均可获得数量较多的DCs。结论较60~70岁组,≥80岁组外周血培养的DCs体外的生长活力、T细胞刺激能力明显降低,可能是≥80岁老年人免疫功能降低的重要原因。

Objective To investigate the culture and immunologic function of the dendritic cells（DCs） derived from the very elderly in vitro. Methods Peripheral blood was collected from the very elderly （ ≥80 years old, n = 30 ） and the elderly （60-70 years old, n = 30）. The mononuelear cells, separated by centrifugalization, were cultured with rhGM-CSF and rhIL-4 for a week, and the growth velocity, vigour, survival time and mixed lymphocytic reaction of DCs were observed. Results In the very elderly, most of the DCs were nonviable at the 7th day after cultured, and no DCs were detected in g subjects. The capacity to stimulate proliferation of allogeneic T lymphocytes was also lower. On the other hand, in the elderly,the DCs grew prosperity in all the subjects. At the 7th day after cultured, DCs could be seen in the every subjects. Conclusions The growth velocity, vigour, survival time, and the capacity to stimulate proliferation of allogeneic T lymphocytes of the DCs are lower in the very elderly than that in the younger elderly, which may be the important reason for the immunological function impairment in the very elderly.