微波辅助萃取法测定烟草中有机氯类农药残留量

Determination of organochlorine pesticides residues in tobacco by microwave-assisted extraction

建立了一种微波辅助萃取．固相萃取净化测定烟草中17种有机氯类农药残留量的新方法。样品用V（正己烷）：V（乙酸乙酯）=1：1提取，提取液经Florisil固相萃取柱净化后，采用气相色谱．电子捕获检测器（GC-uECD）进行检测。17种有机氯农药的0．01、0．05mg／kg和0．5mg／kg加标回收率均在82％以上，RSD在0．11％～8．2％之间，能满足当前烟草中有机氯农药残留的检测要求。

A new method for the determination of 17 organochlorine pesticides （OCPs） in tobacco based on microwaveassisted extraction （MAE） followed by solid-phase extraction （SPE） clean-up is described. Samples were extracted with hexane-ethyl acetate （1：1）, and the extracts were purified by solid-phase extraction using florisil as the adsorbent. Analytes were determined by gas chromatography with electron capture detection （GC-ECD）. The recoveries obtained from samples spiked with standards at the concentration levels of 0.01, 0.05 and 0.5 mg/kg were more than 82%, the relative standard deviations （RSD） were in the range of 0.11% - 8.2%. The method can be used for simultaneous determination of organochlorine pesticide residues in tobacco.