快速分离新生隐球菌RNA

Rapid extraction of RNA of Cryptococcus neoformans

目的：获取新生隐球菌总ＲＮＡ。方法：异硫氰酸胍一步法。结果：成功地获得了高质量的［（１．２３±０．０３）μｇ／１０５细胞］新生隐球菌总ＲＮＡ。结论：将异硫氰酸胍一步法应用于新生隐球菌总ＲＮＡ的抽提，适用于大样本或小样本量的新生隐球菌ＲＮＡ的分离。

Objective: To obtain high yields of RNA of good purity from Cryptococcus neoformans. Methods: A singlestep method of RNA isolation from Cryptococcus neoformans by isoguanidine thiocyanate extraction. Results: High yields of RNA of good purity [(1.23±0.03) μg of RNA from 105 cells] were obtained successfully. Conclusion: The RNA extracted from Cryptococcus neoformans by this method is suitable for use in various molecular applications.