6-磷酸葡萄糖异构酶抗原在类风湿性关节炎中的表达及初步研究

The study and expression of glucose-6-phosphate isomerase in patients with rheumatoid arthritis

目的检测6-磷酸葡萄糖异构酶(GPI)抗原在类风湿关节炎(RA)中的表达情况,探讨其在RA中的诊断价值及临床意义。方法用ELISA法测定116例RA患者5、0例疑似RA患者、114例非RA患者及60例健康人血清中的GPI抗原。比较活动性RA患者与非活动性RA患者的GPI抗原水平。分析GPI抗原水平与类风湿因子(RF)的相关性。结果GPI抗原对RA诊断的敏感性为84.5%,特异性为96.6%;GPI抗原与类风湿因子存在相关性,两者联合检测敏感性增加至90.5%;活动性RA患者与非活动性RA患者GPI抗原水平有显著性差异(P<0.01);不同的X线分期GPI抗原水平有显著性差异(P<0.01)。结论GPI抗原对RA的诊断具有良好的敏感性和特异性,并与RA的活动性及病程进展相关,有助于RA诊断及病情的判断。

Objective To examine the expression of glucose-6-phosphate isomerase （GPI） in patients with rheumatoid arthritis （RA）, and explore its diagnostic value. Methods The GPI was detected by ELISA in 116 cases of RA, 50 cases of suspected patients from RA, 114 non-RA and 60 cases of normal control. The level of GPI between the active and inactive RA patients was compared, and the correlations among the level of GPI and RF vcere analyzed.Results In RA,its sensitivity was 84.5%, speeifieity was 96.6%. GPI was correlated with rheumatoid factor （ RF）. The level of GPI in active RA group was significantly higher than in inactive RA group. The concentration of GPI was significantly different in the patients with different X-ray grades. Conclusion GPI has a reasonable sensitivity and high specificity to diagnosis of RA. GPI positive patients seem to have a more advanced disease process, and GP1 is associated with activity of RA.