Transmission Electron Microscopy Observation of Different Callus Structures in Heiya No.14

黑亚14号不同类型愈伤组织结构的透射电镜观察

[ Objective] The aim was to observe the ultrastructure of different callus structures in Heiya No. 14 by transmission electron microscopy. [Methods] Sample preparation and observation were both carried out by conventional transmission electron microscopy. [ Results] It was showed by transmission electron microscopy that the initial callus cells had a large central vacuole, which squeezed its cytoplasm to be a thin layer around the brim of cell, Meanwhile the nuclear was also squeezed to distribute in the corner of cell, but its nucleolus could be still observed; Compared embryogenic callus with initial callus, its cell wall became thick, and many starch grains and chloroplasts including starch grains could be observed in the cytoplasm area of cell membrane; In non-embryoenic callus, no organelles except for the vacuole could be observed; In browning callus, there was almost no organelles in cells. [ Conclusion] There are significant differences in different types of flax callus at the cell ultrastructure level, which can be as an index for reflecting the differentiation ability of callus cell.

黑龙江省农业科学院经济作物研究所利用外源DNA导入技术，采用柱头基部切割滴注法以法国品种范妮（FANY）为供体，黑亚10号为受体育成了品系D93005—15—8，其各产量性状均超过其受体亲本和当前的主栽品种，2003年由黑龙江省品种审定委员会审定通过，命名为黑亚14号（H14）。