猪水肿病大肠杆菌FedF的B细胞抗原表位预测

Prediction of the B Cell Epitope for the FedF of Esherichia coli Eliciting Edema of Piglet

目的是预测致猪水肿病主要毒力因子FedF的B细胞抗原表位。方法为基于FedF的蛋白基因组序列,采用Garnier-Robson法、Chou-Fasman法和Karplus-Schulz法预测其结构蛋白的二级结构柔性区域,Kyte-Doolittle方案预测其蛋白的亲水性,Emini方案预测其蛋白结构的表面可能性,Jameson-Wolf方案预测其蛋白结构的抗原指数,同时使用BepiPred在线分析预测其可能的B细胞抗原表位,综合以上方法最终预测FedF可能的B细胞表位。结果显示,经过分析推测FedF最有可能的B细胞表位位于FedF蛋白N端第51-57、115-122、148-153、199-206、225-232、254-259区段内。

Objective To predict the B cell epitope for the FedF of Escherichia coil eliciting edema of piglet. Methods Based on FedF genome sequence, the flexible regions Of secondary structure for FedF protein were predicted by methods of Garnier-Robson, Chou-Fasman and Karplus-Schulz, the hydrophilicity plot of FedF protein were predicted by method of Kyte- Doolittle, the surface probability plot of FedF protein were predicted by method of Emini, and the Antigenicity index of FedF protein were predicted by method of Jameson-Wolf..At the meanwbile, theB cell epitopes for the FedF protein were predicted with the online softwear BepiPred. After having been analyzed by all of the methods above, the final possible B cell epitopes of F-edF protein will be predicted. Results The most possible B cell epitopes for FedF were located within or ＋ nearby its N-terminal No.51-57.115-122.148-153.199-206.225-232.254-259.