大菱鲆(Scophthalmus maximus)生长性状相关的微卫星标记筛选

MICROSATELLITES MOLECULAR MARKERS AND THE CORRELATION TO GROWTH TRAIT OF SCOPHTHALMUS MAXIMUS

采用分群分离分析法,以同一批受精卵孵化的同池养殖大菱鲆生长性状发生分离的群体为材料,进行微卫星DNA遗传分析,以揭示影响大菱鲆生长发育速度方面的遗传信息。30个多态性微卫星位点对生长高值组(F组)和生长低值组(S组)各10个样本建立的DNA混合池进行扩增时,在两池间出现了7个差异片段。通过对两组大菱鲆个体PCR扩增出的差异条带进行统计,分析微卫星位点与大菱鲆生长性状的相关性。结果表明,有1个微卫星位点(SmaC10)在355bp的等位基因片段与大菱鲆生长性状存在一定的负相关性;有4个微卫星位点(SmaC02、SmaC06、SmaC09、B11-I12/6/3)分别在258bp、182bp、226bp、175bp的等位基因片段与大菱鲆生长性状存在正相关性,其中位点Smac09在226bp的等位基因片段与生长性状的正相关性极显著,相关系数达到0.354。位点SmaC09所扩增出的等位基因片段可作为具有良好生长特性人工选育群体的分子标记,指导大菱鲆的辅助育种。

Genetic variation of cultured stock of Scophthalmus maximus L. whose growth trait was segregated was studied with bulked segregant analysis. Two classes with mixed DNA template of 10 samples were analyzed in 30 polymorphic microsatellites loci. A total of 7 unique alleles for each class were found in all the loci. After PCR amplification on the individuals, differential bands were calculated and the correlation between growth trait and microsatellite loci was established. The results show that one locus is negatively correlated with growth trait significantly, while other four loci, positively related, among them SmaC09 is of extreme significance at 0.354 （P〈0.01）. This microsatellite and its differential band could be used as a molecular marker to selected stocks of good growth performance at molecular level for S. maximus.