虎尾轮根化学成分研究

Studies on Chemical Constituents in Roots of Uraria crinita

目的研究虎尾轮(Uraria crinita)根的化学成分。方法采用硅胶、大孔树脂、Sephadex LH-20和ODS等色谱手段进行化学成分的分离纯化;依据理化性质、波谱数据和相关文献进行结构鉴定。结果分离并鉴定了14个化合物,分别为β-谷甾醇(β-sitosterol,1),硬脂酸(octadecanoic acid,2),软脂酸(palmitic acid,3),β-胡萝卜苷(β-daucosterol,4),(24R)-5α-豆甾-7,22(E)-二烯-3α-醇[(24R)-stigmast-7,22(E)-dien-3α-ol,5],2-(乙酰胺基)-苯甲酸甲酯[2-(acetylamino)benzoic acid,methyl ester,6],白桦脂醇(betulin,7),间羟基苯甲酸(m-hydroxybenzoic acid,8),5,7-二羟基-2'-甲氧基-3',4'-亚甲基二氧异黄酮(5,7-dihydroxy-2'-methoxy-3',4'-methylenedioxyisoflavanone,9),蔗糖(sucrose,10),槐二醇(sophoradiol,11),染料木素(genistein,12),染料木苷(genistin,13)和水飞蓟宾(silybin,14)。结论化合物5,6,7,8,9,10,11,13,14为首次从虎尾轮根中得到。

OBJECTIVE To study chemical constituents in roots of Uraria crinita. METHODS The compounds were isolated by means of column chromatography on sinica gel, DIAION HP20, Sephadex LH-20 and ODS. Their structures were elucidated by physicochemical properties and spectra. RESULTS Fourteen compounds were obtained and identified as β-sitosterol （ 1 ）, octadecanoic acid （2）, palmitic acid （ 3 ）, β-daucosterol （4）, （24R）-stigmast-7, 22 （E） -dien-3α-ol （ 5 ）, 2-（acetylamino）benzoic acid, methyl ester （6）, betulin （7）, m-hydroxybenzoic acid （8）, 5,7-dihydroxy-2＇-methoxy-3＇,4＇-methylenedioxyisoflavanone （9）, sucrose （10）, sophoradiol （11）, genistein （12）, genistin （13）, andsilybin（14）.CONCLUSION Compound5, 6, 7, 8, 9, 10, 11, 13, and 14 were isolated from the plant for the firsttime.