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胸部开放伤后海水和淡水浸泡对肺损伤的作用比较 被引量:4

Differences between acute lung injury induced by immersion in seawater and in freshwater in dogs after open chest trauma
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摘要 目的比较胸部开放伤后胸腔海水与淡水浸泡对肺损伤的作用。方法24只实验犬致胸部开放伤后分成对照组、海水组和淡水组。海水组和淡水组伤后10min内经伤口分别灌入海水和淡水;观察胸部开放伤后0、2、4、6、8h的生命体征,测渗透压、血浆炎症因子变化。伤后8h取肺组织观察病理学和炎症因子变化。结果肺泡通透指数:海水组较淡水组明显升高(P<0.05),淡水组与对照组比较无统计学差异(P>0.05);IL-1β、IL-8:海水组较淡水组升高(P<0.05),淡水组较对照组升高(P<0.05);血管性血友病因子(von Willnbrang factor,vWf):海水组较淡水组、对照组升高(P<0.05),淡水组较对照组无差异。相关分析显示:血浆渗透压与肺泡通透性指数、炎症因子IL-1β、IL-8正相关(P<0.05)。结论海水较淡水对开放伤后肺组织的损伤程度更严重,可能与海水高渗加重肺血管通透性和过度炎症反应有关。 Objective To compare the differences between acute lung injury ( ALI) induced by seawaterimmersion and freshwater-immersion after open chest trauma. Methods Twenty-four dogs were randomly divided into 3 groups,ALI model group ( control) ,seawater immersion group ( group SW) ,and freshwater immersion group ( group FW) . ALI model was established by cutting an incision of 0. 5 cm at the 4th intercostal space. Then artificial seawater or pure water ( 35 ml/kg) was perfused into the incision for the later 2 groups. The pathological changes were evaluated by light microscopy,hemotoxin and eosin ( HE) staining in 8 h after perfusion. In 0,,4,6 and 8 h after trauma,the plasma samples were collected to test the levels of IL-1β, IL-8 and von Willnbrang factor ( vWF) with ELISA. The amount of total protein in plasma and bronchoalveolar lavage fluid ( BALF) were measured. Results Compared with group FW,the pulmonary penetration index, the concentrations of IL-1β,IL-8 and vWF in the plasma and lung tissue in group SW were markedly higher. They were significantly positively correlated with the plasma osmotic pressure ( P〉0. 05) . Conclusion Despite immersing in seawater and in freshwater worsen the ALI after open chest trauma,the ALI induced by seawater-immersion is severer than that caused by freshwater-immersion. It is attributed to higher plasma osmotic pressure and abundance of salts after seawater immersion,which increasing the pulmonary penetration index and aggravating the inflammatory response.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2009年第21期2090-2093,共4页 Journal of Third Military Medical University
基金 海军后勤科研计划课题(2004-3304)~~
关键词 急性肺损伤 淡水 海水 胸部开放伤 浸泡 acute lung injury freshwater seawater open chest trauma immersion
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参考文献9

  • 1沈宏亮,王强.海水浸泡创伤病理生理变化和救治措施的研究进展[J].第二军医大学学报,2003,24(12):1365-1367. 被引量:18
  • 2虞积耀,赖西南,李辉,陆松敏,王育红,王正国,鹿尔训,孙笑非,王大鹏.战伤合并海水浸泡生存时间及死亡率的动物实验研究[J].解放军医学杂志,2005,30(3):268-269. 被引量:31
  • 3孟激光,段蕴铀,薛志强,冯华松,彭朝胜,丁新民,姜涛.胸部开放伤后海水灌入胸腔致急性肺损伤犬模型的实验研究[J].中华航海医学与高气压医学杂志,2008,15(2):74-77. 被引量:10
  • 4Haddadel B, McCluskie K, Birrell M A,et al. Differential effects of ebselen on neutrophil recruitment, chemokine, and inflammatory mediator expression in a rat model of lipopolysaccharide-induced pulmonary inflammation [ J ]. J Immunol, 2002, 169 (2) : 974 - 982.
  • 5Guo R F, Ward P A. Mediators and regulation of neutrophil accumulation in inflammatory responses in lung: insights from the IgG immune complex model[J]. Free Radic Biol Med, 2002, 33(3) : 303 -310.
  • 6Riffo-Vasquez Y, Spina D. Role of cytokines and chemokines in bronchial hyperresponsiveness and airway inflammation [ J ]. Pharmacol Ther, 2002, 94(3) : 185 -211.
  • 7Mukaida N. Pathophysiologieal roles of interleukin-8/CXCL8 in pulmonary diseases[ J]. Am J Physiol Lung Cell Mol Physiol, 2003, 284 (4) : L566 - L577.
  • 8Shames B D, Zallen G S, McIntyre R C Jr,et al. Chemokines as mediators of diseases related to surgical conditions[ J]. Shock, 2000, 14 (1): 1-7.
  • 9Varol E, Ozaydin M, Sahin M, et al. vWF levels as a circulating marker of endothelial dysfunction in patients with hypertrophic cardiomyopathy[J]. Indian Heart J, 2005, 57(6): 655-657.

二级参考文献37

  • 1金浩生,吴印爱,刘献棠,王志伟,谢尚奎,罗真东.大鼠腹腔海水浸泡时间与死亡率的关系[J].华南国防医学杂志,2002,16(2):7-8. 被引量:3
  • 2谢培增,何强华,朱红胜,刘君,梁鹿章,李扬,王松青,谢星.35例开放性颅脑伤伴海水浸泡病人的治疗体会[J].中华神经医学杂志,2002,1(1):33-34. 被引量:3
  • 3孟激光,段蕴铀,薛志强,丁新民,彭朝胜,王大鹏.机械通气在胸部开放伤后海水灌入胸腔致急性肺损伤救治中的应用[J].中华航海医学与高气压医学杂志,2006,13(4):193-195. 被引量:5
  • 4中华医学会呼吸病学分会.急性肺损伤/急性呼吸窘迫综合征的诊断标准(草案)[J].中华结核和呼吸杂志,2000,23(4):203-203.
  • 5温德伟 张翠霞.碘酊治疗海水浸泡的软组织外伤[J].中华航海医学杂志,1996,3:59-59.
  • 6Milberg JA, Davis DR, Steinberg KP, et al. Improved survival of patients with acute respiratory distress syndrome(ARDS) :1983- 93. JAMA, 1995,86:273-306.
  • 7Standiford TJ, Kunkel SL, Lukacs NW, et al. Macrophage inflammatorly protein-1 alpha mediates lung leukocyte recruitment, lung capillary leak, and early mortality in murine endotoxemia, lmmunol, 1995,155 : 1515-1524.
  • 8Ashbaugh DG, Bigelow DB, Petty TL, et al. Acute respiratory ditress in adults. Lancet, 1967,2:319-332.
  • 9Arthur PW, Gordon RB. Acute lung injury and the acute respiratory distress syndrome: a clinical review. Lancet,2007, 369 : 1553-1565.
  • 10Shapiro L,Dinarello CA. Hyperosmotic stress as stimulant for proinflammatory cytokine production[J]. Exp Cell Res, 1997.23(2) :354-362.

共引文献52

同被引文献28

  • 1虞积耀,赖西南.海战伤合并海水浸泡伤的伤情特点及救治技术研究进展[J].解放军医学杂志,2004,29(12):1017-1019. 被引量:113
  • 2薛志强,段蕴铀,孟激光,丁新民,彭朝胜.肺保护性通气治疗胸腔海水致急性肺损伤的实验研究[J].第二军医大学学报,2005,26(12):1358-1360. 被引量:5
  • 3薛志强,段蕴铀,孟激光,王伟,王大鹏,丁新民,彭朝胜.己酮可可碱对胸部开放伤海水浸泡犬肺血管通透性的作用[J].中华航海医学与高气压医学杂志,2007,14(1):35-37. 被引量:8
  • 4梁子钧 戴稼禾 编著.体液渗透压测定在医学中的应用[M].北京:人民卫生出版社,1988.420.
  • 5Ye S Q, Simon B A, Maloney J P, et al. Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury [ J ]. Am J Respir Crit Care Med, 2005, 171(4) : 361 -370.
  • 6Tang B M, Craig J C, Eslick G D, et al. Use of corticosteroids in acute lung injury and acute respiratory distress syndrome: a systematic review and meta-analysis[ J]. Crit Care Med, 2009, 37 (5) : 1594 - 1603.
  • 7Kitani T, Okuno S, Fujisawa H. Growlh phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor [ J ]. FEBS Lett, 2003, 544(1 -3): 74-78.
  • 8McGlothlin J R, Gao L, Lavoie T, et al. Molecular cloning and characterization of canine pre-B-cell colony-enhancing tactor[ J ]. Biochem Genet, 2005, 43(3 -4) : 127 - 141.
  • 9Ognjanovic S, Bryant-Greenwood G D. Pre-B-cell colony-enhancing factor, a novel cytokine of human fetal membranes[ J]. Am J Obstet Gynecol, 2002, 187(4) : 1051 - 1058.
  • 10Jia S H, Li Y, Parodo J, et al. Pre-B cell colony-etlhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis[J]. J ClinInvest, 2004, 113(9): 1318-1327.

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