摘要
为了验证传统中药方剂玉屏风散对鸡传染性法氏囊病(IBD)疫苗免疫效果的影响,将玉屏风散以高(A)(1%)、中(B)(0.75%)、低(C)(0.5%)剂量拌料饲喂7日龄雏鸡,连喂12 d;14日龄用法氏囊疫苗免疫,分别于21、28、35、42和49日龄用ELISA法测定外周血IBDV抗体;于21和35日龄用流式细胞术测定外周血CD4+和CD8+T细胞亚群;于21、35和49日龄用碳粒廓清试验测定外周血巨噬细胞的吞噬活性。结果表明,玉屏风散可显著提高血清IBDV抗体水平,28日龄后A组抗体水平显著高于其他各组(P<0.05),35日龄后B、C组抗体水平明显高于对照组(P<0.01);CD4+数量及CD4+/CD8+值在21日龄时A、B组显著高于C组和对照组(P<0.05或P<0.01),35日龄时A组显著高于其余各组(P<0.01);玉屏风散也能提高鸡外周血巨噬细胞的吞噬活性,A组效果最好(P<0.05或P<0.01)。结果提示,玉屏风散可提高雏鸡的特异性体液免疫和非特异性细胞免疫水平。
In order to investigate the effect of the Chinese Herbal Medicine formula Jade-screen (Yu Ping Feng San) on response to infectious bursal disease(IBD) vaccine in chickens, the birds vaccinated with IBD vaccine were feed with Jade-screen Powder at high (A) (1%), middle (B) (0.75%) and low (C) (0. 5%) dosages. And some other birds were allocated to two control group, one was omitted for herbal treatment and the other was omitted for both vaccination and herbal treatment. Peripheral blood samples were taken on 21st , 28th, 35th, 42nd and 49th days from ten chickens in each group for detecting the level of antibody to IBDV. T cell subsets of CD4+ and CD8+ were determined by flow cytometry at the 21~' and 35th days. Activity of phagocytosis of macrophages was determined with the experiment of carbon particles clearance at the 21st , 35th and 49th days. The results suggested that Jade-screen Powder can increase the level of antibody to IBDV. A manifests higher level of the antibody than other groups, and the difference was significant after the 28th day (P〈0.05). B and C have the higher level than the controls after the 35th day (P〈0.01). Jade-screen Powder can increase density of CD4^+T cell and the ratio of CD4^+/ CD8+^ significantly (P〈0.05 or P〈0.01). Jade-screen Powder can also increase the activity of phagocytosis of macrophage, and A has the better effect (P〈0.05 or P〈0.01). In conclusion,Jade-screen Powder can enhance both the humeral and cellular immunity in chickens challenged with IBD vaccine.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第10期1564-1568,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北农业大学科技将帅计划项目(2005-17)
石家庄市科技局重大项目资助(08150132A-2)
关键词
玉屏风散
T细胞亚群
巨噬细胞
吞噬活性
雏鸡
Jade-screen (Yu Ping Feng San) Powder
T lymphocyte subset
macrophage
activity of phagocytosis
chicken