超声强化提取啤酒糟水溶性多糖的工艺研究

Study on Extraction of Water Soluble Polysaccharide from Brewer s Spent Grain

为获得啤酒糟水溶性多糖的工艺条件,采用超声强化法提取啤酒糟多糖,确定最佳工艺条件为超声功率180W、超声温度70℃、超声时间15min、液固比25:1(ml/g),在此条件下多糖的得率为5.79%。经过初步纯化,多糖质量分数达到了92.59%。结果表明,超声可有效强化提取啤酒糟水溶性多糖。

Brewer s spent grain （BSG） contains about 28% （db） water soluble polysaccharide, which could be a good resource of the plant polysaccharide. The extraction of water soluble polysaccharide of BSG under ultrasonic assistance was carried out. The optimum extraction conditions were found at the ultrasonic power of 180 W, extraction temperature of around 70 ℃, 15 min of ultrasonic treatment time, and the ratio of liquid to solid of 25：1 （ml/g）. Under this condition, the yield of polysaccharide was up to 5.79%. Though the preliminary purification, the water soluble polysaccharide from BSG reached 92.59%. The results revealed that the extraction of water soluble plant polysaccharide was efficiently enhanced by ultrasound assistance.