粳稻米碱消值的数量性状基因座检测

Identification of Quantitative Trait Loci for Alkali Digestion Value in Japonica Rice

利用碱消值差异显著的大粒粳稻DL115与小粒粳稻XL005杂交获得的200个单株F2群体为作图群体,采用复合区间作图方法,利用SSR标记进行了粳稻碱消值数量性状基因座的检测。结果表明,在F2群体中碱消值呈正态连续分布,表现为由多基因控制的数量性状。共检测到与碱消值相关的QTL3个,qADV3、qADV5和qADV11,分别位于第3、第5和第11染色体的RM14870～RM1284、RM3838～RM3351和RM1812～RM332区间,对表型变异的解释率分别为6.5%、10.3%和8.1%。qADV5的增效等位基因来自碱消值较小的亲本XL005,qADV3和qADV11的增效等位基因来自碱消值较大的亲本DL115;基因作用方式表现为显性或超显性。

Using an F2 population including 200 individuals derived from a cross combination between two japonica rice DL115 with large grain and XL005 with small grain, which were significantly different on alkali digestion value （ADV）, the quantitative trait loci for ADV were identified by composite interval mapping with SSR markers. The results showed that ADV exhibited a normal continuous distribution in F2 population, indicating that ADV is a quantitative trait controlled by multiple genes. A total of three QTLs conferring ADV were detected in intervals of RM14870-RM1284, RM3838-RM3351, and RMI812-RM332 on chromosomes 3, 5, and 11, and explained 6.5%, 10.3% and 8.1% of the observed phenotypic variance, respectively. The allele of qADV5 was derived from XL005 with less ADV, and the alleles of qADV3 and qADV11 were derived from DLI15 with bigger ADV. The gene action was controlled by dominance and over-dominance.