摘要
目的建立清眩片中欧前胡素和异欧前胡素含量测定的方法。方法采用高效液相色谱法，色谱柱：C18-A色谱柱（伊利特，200mm×4．6mm，5μm）；流动相：甲醇水（55：45）；流速：1．0mL·min^-1;检测波长：300nm。结果欧前胡素和异欧前胡素进样量分别在0．0151～0．1359μg和0．0105～0．0945μg与峰面积具有良好的线性关系（r=0．9999），平均加样回收率分别为99．6％和100．6％，RSD分别为0．56％（n=6）和1．92％（n=6）。结论本方法能够快速、准确、方便的检测清眩片中欧前胡素和异欧前胡素的含量，可作为清眩片质量评价的依据。
Objective To establish an HPLC method for the determination of imperatorin and isoimperatorin in Qingxuan tablets. Methods HPLC method was used. The column was C18-A （200 mm× 4. 6 mm, 5 μm）. The mobile phase was methanolwater （55 ： 45）, the flow rate was 1.0 mL ·min ^-1,and the detective wavelength was 300 nm. Results The linear range of imperatorin and isoimperatorin was 0. 015 1-0. 135 9 and 0. 010 5-0. 094 5μg. The average recovery was 99. 6% and100.6%, withRSD=0.56% （n=6）andRSD=1.92% （n=6）. Conclusion The method can be used to detect imperatorin and isoimperatorin in Qingxuan tablets rapidly, accurately and conveniently, and control the quality of Qingxuan tablets.
出处
《中南药学》
CAS
2010年第1期30-33,共4页
Central South Pharmacy
