摘要
The efficacies of some indigenous herbal dyes for use in staining plant materials were examined to obtain non-toxic, eco-friendly and cheap stains for use in plant histology. Dye extracts from Bixa orellana, Curcuma domestica, Lonchocarpus cyanescens and Pterocarpus osun were used to stain wood sections using the existing standard staining procedures with little modification. All the extracts had affinity for the fibre and vessel elements except the extract from L. cyanescens. The extracts from C. domestica and B. orellana had higher selectivity than those ofP. osun for fibre. From the results of the absorbance curves, each of the dye extracts from all speciese had minimum of two peaks, indicating that they had two or more colour imparting chromophores except dye extract from C. domestica. All the dye extracts were acidic with pH range of 3.77 to 6.77. Therefore, this study shows that dye extracts from B. orellana, C. domestica and P. osun could be solitarily or in combination with artificial dyes for plant histological staining.
为了改良有毒的、昂贵的、人造的非本地的染剂,研究用于植物组织染色的一些无毒和环保型的草本植物染剂的功效。采用常规的染色方法,利用从红木(Bixa orellana)、姜黄(Curcuma domestica)、约鲁巴靛蓝(Lonchocarpus cyanescens)和紫檀(Pterocarpus osun)中提取的染色剂用于木本植物切片的着色。除了从约鲁巴靛蓝中提取的染色剂外,所有从植物中提取的染色剂都对纤维和导管分子有亲和性。从姜黄和红木中提取的染色剂比从紫檀中提取的染色剂有更高的选则性。吸光率曲线结果表明,除了从姜黄中提取的染色剂外,从植物中提取的染色剂有两个小峰值,因此他们有两个或更多的染色团。染色剂显酸性(pH,3.77～6.77)。研究表明,从红木,姜黄和紫檀中提取的染色剂可以单独或与人工染剂结合用于植物组织染色。图3表2参12。
