人胚胎心肌组织发育过程中Cx43和Bcl-2蛋白表达的研究

Cx43 and Bcl-2 expressions in myocardial tissue during the developmental periods of human fetus

目的观察连接蛋白43(Cx43)和Bcl-2蛋白在人胚胎心室肌组织中的表达,探讨其与心脏发育的关系。方法应用免疫组织化学法检测第2、3、4三个月胎龄段Cx43和Bcl-2蛋白在人胚胎心室肌组织中的表达。结果第2月胚龄时,人胚心室肌组织内可见单个散在分布的Bcl-2阳性细胞,随着胎龄的增加,Bcl-2阳性细胞数量逐渐增多,到第4月胎龄时,在心室肌组织内可见三五成群分布的Bcl-2阳性细胞。第2月胚龄时,Cx43蛋白呈斑点状散在分布于心室肌细胞的细胞质,随着胎龄的增加,其分布范围逐渐扩大,在心室肌细胞与心室肌细胞连接处也出现Cx43蛋白的阳性表达,到第4月胎龄时,Cx43蛋白在心室壁内逐渐形成成片弥散样分布。结论Cx43和Bcl-2蛋白参与了人胚胎早期心室肌组织细胞的生长发育。

Objective To study the expressions of Connexin43 （Cx43） and B-cell lymphoma/leukemia-2 （Bcl-2） proteins in the ventricular myocardium of human fetus,and explore the correlation between the expression of the two proteins and the development of fetus myocardium.MethodsThe expressions of Cx43 and Bcl-2 protein in the ventricular myocardium of human embryos were immunohistochemically detected in the second,third and fourth month of gestation,respectively,by strepto-avidin-biotin-peroxidase complex （SABC） and avidin-biotin-peroxidase complex （ABC） technics.ResultsThe positive expression of Bcl-2 protein was detected in one or two cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the number of Bcl-2 positive cells increased gradually and distributed in clusters in the ventricular myocardium of human fetus in the fourth month of gestation.The cells with positive expression of Cx43 protein were detected and scattered in dots-like structure in the cytoplasm of myocardial cells in the ventricular myocardium of human embryos in the second month of gestation.With the advancement of fetal age,the distributing region of Cx43 protein extended gradually,and the Cx43 protein positively expressed in the membranes between ventricular myocardial cells.The positively expressed Cx43 protein showed a diffused distribution in a sheet form in the ventricular wall of human fetus in the fourth month of gestation.ConclusionCx43 and Bcl-2 proteins participate in the early growth and development of the ventricular myocardial cells of human fetus.