Hedgehog信号通路相关基因在大肠癌的表达及其临床意义

Expressions of Hedgehog Signaling Pathway Related Genes in Colorectal Carcinoma and Their Significances

目的:研究Hedgehog信号通路相关基因SHH、SMO、GLI_1在大肠癌中的表达及其临床意义。方法:用RT-PCR检测43例大肠癌切除标本的癌组织和距离癌10 cm以上的癌旁组织的SHH、SMO、GLI_1 mRNA表达的阳性率,并与20例非肠癌患者的正常大肠组织对照,分析其与大肠癌患者临床和病理各变量的相关性。结果:大肠癌组织SHH mRNA表达阳性率为27.9%,与癌旁组织的18.6%比较,差异无显著性(p>0.05),但显著高于正常组织的0%(P<0.05)。大肠癌组织SMO、GLI_1 mRNA表达阳性率分别为83.7%、34.9%,显著高于癌旁组织的58.1%、16.3%及正常组织的0%、0%(P<0.05)。SHH、SMO、GLI_1 mRNA表达阳性率之间并无相关性,阳性率与患者的年龄、性别、临床分期、肿瘤部位、肿瘤浸润深度、病理分型等变量均无相关性(P>0.05)。结论:Hedgehog信号通路相关基因SHH、SMO、GLI_1 mRNA在大肠癌组织中表达阳性率显著增高,其表达与临床和病理各变量不相关。

Objective：We evaluated the expressions of Hedgehog signaling pathway related genes SHH,SMO, GLI, in colorectal carcinoma （ CRC ） tissues in order to explore their significances in CRC pathogenesis. Methods ： Fresh CRC tissues and matched adjacent tissues （ 10 cm beyond the cancer margin） were collected from 43 confirmed CRC patients undergoing surgical operations. Normal colorectal tissues from 20 non-CRC cases were also collected as controls. The expressions of SHH ,SMO,GLI, mRNA were detected by RT-PCR. The correlation of their positive rates to the clinicopathological characteristics was analyzed. Results：The positive rate 27.9% of SHH mRNA expression in CRC tissues did not statistically differ from that of 18.6% in the adjacent tissues （P 〉 0.05 ） , but was statistically higher than that of 0% in normal colorectal tissues （P 〈0.05）. The positive rates of SMO,GLI1 mRNA expressions in CRC tissues （83.7% ,34.9% ） were statistically higher than those of 58.1% and 16.3% in the adjacent tissues and those of 0% ,0% in normal colorectal tissues （P 〈 0.05 ）. No significant correlation was found among the SHH,SMO, GLI1 mRNA expressions and the expressions to the clinical variables such as age ,gender,Dukes stages ,tumor locations, depth of tumor invasion and histological types of CRC （ P 〉 0. 05 ）. Conclusions ： The positive rates of Hedgehog signaling pathway related genes SHH, SMO, GLI1 mRNA expressions increased significantly in CRC tissues. The high expressions were not related to the clinical and pathological indexes of severity.