口腔疣状癌伴灶性鳞癌颈淋巴结转移灶裸鼠移植瘤模型的建立

Establishment of xenografted model of oral verrucous carcinoma with focal squamous cell carcinoma metastasizing to the cervical lymph nodes in nude mice

目的:建立口腔疣状癌伴灶性鳞癌颈淋巴结转移灶移植瘤原代模型,探讨建立模型的方法。方法:将口腔疣状癌伴灶性鳞癌颈淋巴结转移组织,通过组织块皮下接种法建立颈部淋巴结转移灶模型。观察其生长状况,对移植瘤、裸鼠肝、肾、肺及淋巴结行系列检查并与来源肿瘤组织进行比较。结果:成瘤率87.5%(7/8),移植瘤具有来源肿瘤组织类似的形态学特征,肝、肾、肺未见转移,腋下及腹股沟淋巴结反应性增生。结论:通过组织块皮下接种法,成功建立了口腔疣状癌伴灶性鳞癌颈淋巴结转移灶移植瘤原代模型。

PURPOSE： To use the excised cervical lymph node tissue from oral verrucous carcinoma patient with focal squamous cell carcinoma subcutaneously to establish a xenografted model in nude mice. METHODS： The xenograft tumors were finally removed for histopathological study and the mice were laparotomized to examine metastatic tumors in livers, kidneys, lungs. RESULTS： The tumor formation rate was 87.5%（7/8）,and the appearance of transplanted tumors was like that in human and HE staining showed that the cancer cells of those tumors models and mesenchymal components remained morphologically like the original tumor. The liver, renal, lung and lymph nodes didn’t show obvious metastasis. CONCLUSION： The xenografted model is successfully established with a higher formation rate, and the model morphologically resembles the human tumor.