HPLC-ELSD法测定黄芪及金芪降糖片中黄芪甲苷的含量

Determination of astragaloside Ⅳ in Radix astragali and Jinqi jiangtang tablet by HPLC-ELSD

目的:建立黄芪及金芪降糖片中黄芪甲苷的测定方法。方法:采用高效液相色谱-蒸发光散射法(HPLC-ELSD)测定黄芪甲苷的含量。色谱柱为Diamonsil C18(4.6mm×250mm,5μm),以乙腈-水(34:66)为流动相,流速为1.0ml/min,柱温为35℃,漂移管温度为105℃,载气流量为2.5L/min。结果:黄芪甲苷进样量在2.056～8.224μg范围内具有良好的线性关系(r=0.9995),黄芪药材中黄芪甲苷的平均回收率为98.67%(RSD=1.72%),金芪降糖片中黄芪甲苷的平均回收率为98.13%(RSD=1.34%)。结论:该方法操作简便、灵敏度高、专属性强、重现性好,可用于黄芪药材及金芪降糖片的质量控制。

Objective： To establish a quantitative method for determing astragaloside Ⅳ in Radix astragali and Jinqi jiangtang tablet. Methods： The content of astragaloside IV was determined by high- performance liquid chromatogramphy with evaporative light-scattering detection （HPLC -ELSD）. The separation was performed on a Diamonsil Cls column （4.6 mm×250 mm,5 μm） with acetonitrile-water（34： 66） as mobile phase at a flow rate of 1.0 ml/min. The column temperature was 35℃ and drift tube temperature was 105 ℃.The carrier gas flow rate was 2.5 L/min. Results： The linear range of astragaloside IV was 2.056-8.224 μg （r=0.999 5）.The average recovery rate of astragaloside IV was 98.67% （RSD = 1.72%） in Radix astragali and 98.13% （RSD =1.34%） in Jinqi j iangtang tablet respectively. Conclusion： The method is simple, the quality of Radix as sensitive and specific with a good repeatability. The method can be used to control tragali and Jinqi jiangtang tablet.