双波长比值光谱法测定复方氨林巴比妥注射液三组分的含量

Determination of Three Components in Ammonia Lin Phenobarbital Injection Using Dual Wavelength Ratio Spectrometry

依据巴比妥、氨基比林、安替比林三组分的比值光谱特征,对其含量进行了测定.以安替比林为干扰组分,选择227 nm、242 nm作为测定氨基比林的波长;以氨基比林为干扰组分,选择239 nm、255 nm作为测定巴比妥的波长;以巴比妥为干扰组分,选择232 nm、245 nm作为测定安替比林的波长.结果显示氨基比林在1－65 mg.L-1,巴比妥在1.8－116 mg.L-1,安替比林在1－70 mg.L-1范围,ΔR-c具有良好的线性关系.

We determinate the component content in Ammonia Lin Phenobarbital Injection using dual wavelength ratio spectrometry.For arninopyfine,we set the wavelength 227 nm and 242 nm,and others successively are in bartial（239 nm,255 nm）,antipyrine（232 nm,245 nm）.Results indicate that ΔR-c follows linear relationship in the range of 1-65 mg for arninopyfine,1.8-116 mg for barbital and 1-67 for antipyrine.