摘要
以Tris-HC l缓冲溶液作为研究介质,在pH=6.75的环境下,应用电子吸收光谱和荧光光谱法探讨了苏木素(HE)和铒(Ⅲ)形成的配合物Er(Ⅲ)(HE)3与鲱鱼精DNA的作用方式,并用摩尔比法和双倒数法求得金属与配体的结合比为1:3,Er(Ⅲ)(HE)3配合物与鲱鱼精DNA的结合常数为Kθ25℃=1.87×104L/mol。以吖啶橙(AO)作为分子探针探测了Er(Ⅲ)(HE)3配合物与DNA的作用情况,发现配合物能使AO-DNA体系发生动态荧光猝灭,Er(Ⅲ)(HE)3配合物与AO对鲱鱼精DNA具有嵌插竞争作用。
The Tris-HCl buffer solution was used to create acidity environment pH = 6.75 in the study. The interaction between the complex Er（ Ⅲ） （HE）3 and herring-sperm DNA was discussed by UV action spectrum and fluorescence chromatography. In addition, the coupling ratio between Er（ Ⅲ） and HE （ n = 1：3） and the binding constant between Er（ Ⅲ） （ HE）3 and herring-sperm DNA（ K^θ 25 ℃： = 1 87 × 10^4 L/ mol） were also obtained in the study by double unity wavelength substantial amount ratio method and double reciprocal method. AO was used as molecular probe to discuss the interaction between the Complex Er（ Ⅲ ）（HE）3 and DNA. The results show that the complex Er（ Ⅲ） （HE）3 could make AO-DNA composite fluorescence dynamic quench, and the intercalation action competition with herring-sperm DNA exists between AO and Er（ Ⅲ） （HE） 3.
出处
《西南科技大学学报》
CAS
2010年第1期19-23,共5页
Journal of Southwest University of Science and Technology
基金
国家高技术研究发展计划(863计划)资助(2007AA×××133)
国家自然科学基金资助(30572254)
