裕民无刺红花染色体制片优化及核型分析

Optimization of Chromosome Sectioning and Karyotype Analysis of Carthamus tinctorius L.

比较了不同预处理和解离方法对裕民无刺红花染色体制片的影响,观察500个根尖细胞,比较不同部位中期细胞和适宜核型分析的中期细胞所占比例。结果表明:用0.002 mol/L8-羟基喹啉与0.1%秋水仙素按1∶1混合8 h,1 mol/L盐酸常温解离6 min制片所得染色体分散效果最佳。核型公式为2n=2X=24 m,核型不对称系数(As.k%)为58.25%,属于1 B类型,核型对称性程度高,表明裕民无刺红花在进化中处于比较原始类型。

The different methods of the pretreatments and dissociations in chromosome sectioning of Carthamus tinctorius L. were compared. 500 top of root cells were observed, the proportions of metaphase cells and the metaphase cells which were suitable for karyotype analysis were compared. The results showed that the pretreatment with the mixture of 0. 002 mol/L 8-Hydroxyquinolin and 0. 1% Colchicine（v ： v=1：1） 8 h, 1 mol/L HCI at normal temperature 6 rain performed well in the sectioning. The karyotype analysis revealed that the karyotype of Cartharnus tinctorius L. was 2n=2X=24=24m and its index of AS. K% was 58. 25%, the karyotype asymmetry belonged to 1 B type, its karyo- type was symmetrical. This suggested that it was relatively primitive forms.