摘要
目的:研究血管内皮生长因子-C在胃癌中的表达情况,探讨其与胃癌淋巴管形成和淋巴结转移的关系。方法:应用RT-PCR检测60例胃癌患者手术切除新鲜胃癌及癌旁组织中血管内皮生长因子-C mRNA表达情况,免疫组织化学法检测血管内皮生长因子-C蛋白表达情况和微淋巴管密度。结果:60例患者胃癌及癌旁组织中均有血管内皮生长因子-C mRNA表达,胃癌组织较癌旁组织有更高量的表达(P<0.01)。胃癌、癌旁组织中的血管内皮生长因子-C蛋白表达阳性表达率分别为51.67%(31/60),18.33%(11/60)(P<0.01)。血管内皮生长因子-C mRNA及血管内皮生长因子-C蛋白高表达与胃癌淋巴管浸润、淋巴结转移密切相关。结论:血管内皮生长因子-C对胃癌淋巴管形成和淋巴结转移有重要作用。
Objective To study the expression of VEGF-C in gastric carcinoma and its relationship with the formation of lymphatic vessel and lymph node metastasis.Methods The expression of VEGF-C mRNA in 60 pairs of fresh primary gastric cancer and their adjacent normal tissues were determined with semi-quantitative reverse RT-PCR,the expression of VEGF-C protein and lymphatis vessel density in gastric carcinoma tissues were detected with immunohistochemistry.Results VEGF-C mRNA was expressed in 60 pairs of gastric cancer and their adjacent tissues,and gastric cancer tissues had a higher expression than the adjacent tissues（P〈0.01）.The expression rates of VEGF-C protein in gastric carcinoma tissues and the adjacent tissues were 51.67% and 18.33%,which showed a statistic difference（P〈0.01）.The high expressions of VEGF-C mRNA and VEGF-C protein were closely related to lymphovascular invasion and lymphatic metastasis.Conclusion VEGF-C plays an important role in the formation of lymphatic vessel and lymph node metastasis in gastric cancer.
出处
《中华实用诊断与治疗杂志》
2010年第5期433-435,439,共4页
Journal of Chinese Practical Diagnosis and Therapy
