中国特有植物大血藤的RAPD分析──植物地带性分化的分子差异标准初探

RAPD Analysis of Sargentodoxa cuneata and Discussing on the Molecular Divergence of Plant Zonation Differentiation

采用随机扩增多态性ＤＮＡ技术对中国特有科特有属植物大血藤（Ｓａｒｇｅｎｔｏｄｏｘａｃｕｎｅａｔａ）进行分析，探讨植物地带性分化的分子差异．从１００个随机引物中筛选出１１个有效的１０碱基对寡聚核糖核苷酸作引物，用于对取自５省区的大血藤植物基因组ＤＮＡ进行ＰＣＲ扩增；用ＵＰＧＭＡ法对各居群间的Ｊａｃａｒｄ相似性系数和遗传距离进行聚类分析．ＲＡＰＤ分析结果表明：分布于各省区（地区）的大血藤居群，尽管其形态特征的变异尺度较为一致，但在分子水平上存在明显差异，这种差异与地理范围的变化相关，表现出一定的地带性分化规律．

The random amplified polymorphic DNA (RAPD)is often used to solve problems of plant taxonomy and systematics.The purpose of the study is to discuss and demonstrate the molecular characteristics of the zonation differentiation of plants Plant leaves of Sargentodoxa cuneata (Oliv ) Rehd et Wils from 5 provinces and/or regions were collected and eleven 10 mer oligonuclear primers were selected from one hundred primers to amplify plant genomes by RAPD.The genetic data were analyzed by means of similarity and genetic distance,and a phenogram was constructed by using UPGMA method These results showed that the variation in morphological characteristics of Sargentodoxa was conspicuous; and that the variational ranges from different provinces were identical or similar, but there exists high molecular divergence and the divergence tends to be related to the geographical localities where the samples were collected, which may indicate that the differentiation among a lot of populations may have happened in Sargentodoxa due to geographical barriers, ecological barriers and/or the existing of evolutional potentiality in the relic species It also indicates that RAPD method can be applied to floristic study