摘要
在共轭亚油酸高效液相色谱法分析中,通过单因素试验和响应面分析法,研究了NaOCH3-CH3OH甲酯化分析奶样中CLA的最佳条件。在单因素实验的基础上,以Box-Behnken响应面分析法(三因素三水平)优化甲酯化条件。实验结果表明:最适衍生化条件为NaOCH3-CH3OH添加量为8.56mL,温度为24.4℃,时间为12.8min。并以该条件对液态奶中的9c,11t-CLA含量进行了测定。
In this paper, the base-catalyzed conjugated linoleic acid methyl ester preparation was evaluated by the response surface methodology （RSM）. Based on the single factor experiment, the optimum methylation conditions were obtained with Box-Behnken design （BBD）. The results showed that the optimum conditions for the methylation of conjugated linoleic acid were as follows： reaction temperature 24.4℃ , reaction time 12.8 min, 0.5 M sodium methoxide-methanol 8.56 mL for methytation.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2010年第4期96-100,共5页
Food and Fermentation Industries
关键词
共轭亚油酸
高效液相
甲酯化
响应面
conjugated linoleic acid, HPLC, methylation, response surface methodology
