雷公腾多甙对实验性变态反应性神经炎周围神经的影响

The influence of tripterygium polyglucoside intervention on peripheral nerves in experimental allergic neuritis

目的:了解雷公腾多甙(Tripterygium polyglucoside,TPG)对实验性变态反应性神经炎(experimental allergic neuritis,EAN)周围神经的影响。方法:在模型大鼠出现EAN临床症状后连续给予TPG14d,观察EAN临床症状、周围神经电生理与组织病理的变化。结果:免疫后23d EAN坐骨神经的运动神经传导速度和复合肌肉动作电位(compound muscle action potential,CMAP)的波幅明显降低,潜伏期和时限显著延长(P<0.05,与对照组比较)。大量炎性细胞侵入坐骨神经,多数神经纤维呈现严重的髓鞘脱失和相当程度的轴索变性;位于郎飞结间隙和近结旁段的轴膜钠、钾离子通道基本检测不到(P<0.05,与对照组比较)。TPG干预显著减轻了CMAP波幅的降低和潜伏期与时限的延长,坐骨神经炎性反应和脱髓鞘减轻,部分轴膜钠、钾离子通道得以保留(P<0.05与EAN组比较)。结论:TPG作为一种可能减少格林-巴利综合征持久性神经功能损害的药物值得进一步研究。

Objective：To investigate the effect of Tripterygium polyglucoside（TPG）on peripheral nerves in experimental allergic neuritis（EAN）.Methods：TPG was administrated for 14 days in EAN rats after the symptom emerging.The alteration of clinical presentation,electrophysiology and histopathology of peripheral nerves in EAN was observed.Results：Motor nerve conduction velocity and amplitude of compound muscle action potential（CMAP）of the sciatic nerves from EAN decreased significantly along with prolongation of CAMP latency and duration on day 23 post immunization（P〈0.05 vs control group）.The majority of the sciatic nerves was invaded by numerous inflammatory cells and presented severe demyelination and modest axonal degeneration.Few axolemma sodium and potassium channels could be detected in Ranvier nodal gaps and juxtaparanodes of the single fibers（P〈0.05 vs control）.TPG intervention had notable effects on alleviating the CAMP amplitude decrease and prolongation of latency and duration of the sciatic nerves.TPG also significantly reduced inflammation and demyelination of the sciatic nerves and partly alleviated the loss of axolemma ion channels in the fibers（P〈0.05 vs EAN group）.Conclusion：TPG may be a promising agent for reducing the persistent neurological deficits in Guillain-Barré syndrome and worthy of further exploration.