从NO、NOS变化探讨瓜蒌薤白白酒汤对心肌缺血再灌注损伤的防治作用

Research the Control Effect of Injury Caused Ischemia Myocardium on Liquor Gualouxiebai Decoction from the Change of NO and NOS

目的:研究瓜蒌薤白白酒汤(LGXD)对家兔心肌缺血再灌注损伤模型血清中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的影响,并探讨其对再灌注损伤的可能性保护机制。方法:采用结扎左冠状动脉前降支法复制心肌缺血再灌注损伤模型,分离血清检测血清中一氧化氮(NO)含量和一氧化氮合酶(NOS)活性,摘取左室心肌做常规病理切片。结果:与假手术组比较,模型组NO含量和NOS活性均显著增高,与模型组比较,高、低剂量药物组NO含量和NOS活性均降低,低剂量组比高剂量组低,病理结果显示低剂量组优于高剂量组。结论:瓜蒌薤白白酒汤对家兔心肌缺血再灌注损伤的心肌有保护作用,其机制可能与抑制NOS的活性,减少NO的过量产生有关,且低剂量药物组疗效优于高剂量组。

Objective： To study the effects of Liquor Gualouxiebai Decoction NO、NOS Change of Injury Caused Ischemic Myocardium and explore the mechanism of its possible protection to ischemic myocardium.Methods： Ischemic Myocardium was produced by ligating left coronary artery,examine the amount of NO and NOS in the serum,take the injured heart muscle to make biopsy.Result：Compared with sham operation group,NO and NOS in model group were significantly higher,compared with model group,NO and NOS in high-dose and low-dose group were lower,and low-dose group is lower than high-dose group.Pathological result showed that low-dose group was better than the high dose group.Conclusion：Liquor Gualouxiebai Decoction has a protective effect on rabbit ischemic myocardium,the mechanism may be related to the inhibition of NOS activity and to the reduction of excessive production of NO,and low-dose treatment group is more effective than high dose group.