超声波提取银杏叶中黄酮类化合物的研究

Study on extracting Flavonoids from the Leaves of Ginkgo Biloba by Ultrasonic

目的:研究采用超声波法提取银杏叶中黄酮类化合物的工艺。方法:采用正交试验确定银杏叶中总黄酮的最佳提取工艺;以总黄酮为评价指标,用分光光度法进行分析检测,并与传统的溶剂提取法进行比较。结果:最佳提取条件为超声波功率100%,超声时间45min,液固比为12:1,溶剂浓度30%,提取次数2次。分析检测显示,总黄酮的提取率达1.2%,超声提取的提取率比溶剂法提高了约1.5倍。结论:超声波用于银杏叶提取具有成本低、回收率高、有机溶剂残留少等优点。

Objective：To investigate the extraction of flavonaids from the Leaves of Ginkgo Biloba by Ultrasonic.Methods：The extracting congditions of flovonoids from the Leaves of Ginkgo Biloba were optimized by orthogonal design.Spectrophotometry was used to detemine the congtent of flavonids.Results：The optimal extracting conditions were under the 100% ultrasonic power,ultrasonic time was 45 min,the ratio of solvent to feedstock was 12：1,alcohol concentration was 30%,extraction 2 times.The rate of extraction was up to 1.2% by detemine,the yield of ultrasonic extraction was higher about 1.5 fold than that of ethanol-resin adsorption method.Conclusion：This extraction process merits have the advantage of low cost,high recovery and low retained level of organic solvent.