花生AhLEA18蛋白基因的克隆与表达分析

Cloning and Expression Analysis of Peanut AhLEA18 Protein Gene

LEA蛋白家族是一类在种子胚胎发育晚期丰富表达的蛋白,在种子的正常发育过程中大量积累,同时在植物处于干旱、寒冷、盐胁迫等逆境条件下高水平表达,推测该类蛋白在缺水条件下具有一定的生理保护功能。根据蛋白序列特点,该蛋白家族分为多个组,LEA18蛋白属于其中一组蛋白。PvLEA18蛋白在干燥种子、花粉粒中大量积累,也参与到对水分缺失、ABA处理的生理应答过程。该文从栽培种花生鲁花14中克隆得到一个LEA18基因,命名为AhLEA18。对其序列的生物信息学分析表明,AhLEA18具有典型LEA18蛋白序列特征。通过半定量RT-PCR的方法对该基因在花生植株不同组织及不同发育时期的种子中的表达情况进行了分析。

In plants, a group of hydrophilic proteins, known as Late Embryogenesis Abundant （LEA） proteins, accumulate to high level during the late stage of seed development and during water deficiency in vegetative organs, suggesting a protective role during water limitation. LEA18 protein is a member of LEA protein family. ThePv LEA18 transcript and protein are highly accumulated in dry seeds and pollen grains and also respond to water deficit and ABA treatments. One LEA18 protein gene from peanut （Luhua 14） was cloned and named AhLEA18 . Aligning with several LEA18 proteins from other species by ClustalW program, the result demonstrated that theAh LEA18 is a typical LEA18 protein. The expression of this gene in different tissues and four developmental stages of peanut seeds were analyzed by Semi-quantitative RT-PCR.