迟缓爱德华氏菌(Edwardsiella tarda)产生的二聚和三聚吲哚类生物碱

Bis-and Tris-Indole Alkaloids from Edwardsiella tarda

为了开发利用病原菌资源,研究了迟缓爱德华氏菌(Edwardsiella tarda)的次生代谢产物,采用凝胶柱色谱、反相高效液相色谱等分离纯化手段,从该菌发酵产物的乙酸乙酯提取物中分离得到5个吲哚类生物碱1-5。结合其理化性质和波谱学特征,化合物1-5的结构依次鉴定为:bis(1H-indol-3-yl)phenylmethane(1)、1,1-bis(1H-indol-3-yl)-2-phenylethane(2)、(2S)-3,3-bis(1H-indol-3-yl)propane-1,2-diol(3)、1H,1′H,1′′H-3,2′:3′,3′′-terindole(4)和(3,2′:2′,3′′-terindo-lin)-3′-one(5),其中化合物1、2和4为新天然产物。利用药敏纸片法、二倍稀释法和SRB法,对5个单体化合物的抗菌和细胞毒活性进行了初步评价。其中化合物1和3对产气杆菌有微弱的抑制作用,最小抑菌浓度(MIC)均为0.125g/L。

In order to investigate the potential of pathogens for drug discovery,the secondary metabolites of Edwardsiella tarda were studied.As a result,five indole alkaloids were isolated by column chromatography over silica gel,Sephadex LH-20,and HPLC preparation.By spectroscopic analysis,their structures were identified as bis（1H-indol-3-yl）phenylmethane（1）,1,1-bis（1H-indol-3-yl）-2-phenylethane（2）,（2S）-3,3-bis（1H-indol-3-yl）propane-1,2-diol（3）,1H,1＇H,1＇＇H-3,2＇：3＇,3＇＇-terindole（4）,and（3,2＇：2＇,3＇＇-terindolin）-3＇-one（5）,respectively.To the best our knowledge,compounds 1,2 and 4 were isolated as the natural products for the first time.Both compounds 1 and 3 showed weak anti-bacterial activity against Clostridium perfringens with MIC values of 0.125 g/L.