OX40/OX40L mRNA在实验性变态反应性神经炎中的动态变化

Dynamic change OX40/OX40L mRNA in experimental allergic neuritis

目的:研究Oxford 40(OX40)和Oxford 40 ligand(OX40L)mRNA在实验性变态反应性神经炎(experimental allegic neuritis,EAN)大鼠坐骨神经、脾脏、外周血和淋巴结中的动态变化。方法:36只Lewis大鼠随机分为EAN模型组和完全弗氏佐剂对照组(CFA组)。分别在第9天、第17天、第26天处死动物,采用反转录聚合酶链反应技术检测坐骨神经根、脾脏、外周血单个核细胞和淋巴结中OX40和OX40LmRNA的表达水平。结果:EAN组大鼠在抗原免疫后第17天达到发病高峰,OX40和OX40LmRNA在第9天(发病早期)和第17天时表达均较高,与第26天(恢复期)相比,差异有统计学意义(P<0.05),各时间点与对照组相比差异有统计学意义(P<0.05);CFA组大鼠无症状;EAN组中OX40和OX40LmRNA在坐骨神经和淋巴结中各时间点表达均升高,在外周血单个核细胞中微量表达。结论:OX40/OX40L可能与EAN发病有关。

Objective To examine the expression of mRNA of Oxford 40（OX40） and Oxford 40 ligand（OX40L） in the sciatic nerve,spleen,peripheral blood mononuclear cells and lymph nodes of experimental allegic neuritis（EAN）.Methods Thirty-six Lewis rats were randomly assigned into an EAN group and a CFA group.The rats were sacrificed on 9th,17th,and 26th day after immunization.OX40 and OX40L mRNA was detected by reverse transcription polymerase chain reaction in the spleen,sciatic nerves,peripheral blood mononuclear cells and lymphonodes.Results The peak of clinical course came on 17th day after the immuuization in EAN.The mRNA expression of OX40/OX40L was higher on 8th day and 17th day than that on 26th day after the immuuization（P〈0.05）.There was significant difference between the EAN group and the CFA group at the 3 time points（P〈0.05）;rats in the CFA group didn＇t have any clinical manifestations.The mRNA expression of OX40 and OX40L in the EAN group raised in the sciatic nerves and lymph nodes at the above 3 time points（P〈0.05）.Weak expression was seen in the peripheral blood mononuclear cells.Conclusion OX40 and OX40L may play a role in the pathogenesis of experimental allegic neuritis.