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大鼠脂肪源性干细胞和骨髓间充质干细胞增殖速度的比较性研究 被引量：2

THE DIFFERENCE BETWEEN PROLIFERATION RATE OF RAT ADSC AND ANBMMSC
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摘要 目的:比较ADSC和BMMSC在增殖速度上的差异性,初步探讨二者的优越性。方法:应用酶消化分离法分离AD-SC,差速贴壁法分离BMMSC,MTT法比较增殖速度。结果:第一代和第三代都呈现ADSC在培养48h后比BMMSC增殖快,P<0.05。结论:ADSC增殖快于BMMSC,体外扩增更容易快速获得大量种子细胞。 Objective：To compare the proliferation rate of rat ADSC and BMMSC;to approach the superiority.Methods：Isolate the ADSC by enzyme digestion;isolate the BMMSC by differential velocity adherent technique;compare the proliferation rate of rat by MTT.Results： ADSC proliferated faster than BMMSC after 48 hours at genenation 1 and genenation 3.Conclusion： ADSC proliferated faster than BMMSC.
作者 秦俭 董嘉楠 赵微
机构地区 牡丹江医学院解剖教研室
出处 《牡丹江医学院学报》 2010年第4期21-23,共3页 Journal of Mudanjiang Medical University
关键词 ADSC BMMSC 大鼠 增殖速度 ADSC BMMSC rat proliferation rate
分类号 R322 [医药卫生—人体解剖和组织胚胎学]
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参考文献5

  • 1K.G.Gaustad,A.C.Boquest,B.E.Anderson,A.M.Gerdes,P.Collas,Differentiation of human adipose tissue stem cells using extracts of rat cardiomyocytes,Biochem.Biophys.Res.Commun.314 (2004):420–427.
  • 2Zuk PA,Zhu M,Ashjian P et al.Human adipose tissue is a source of multipotent stem cells.Mol Biol Cell,2002,13:4279–4295.
  • 3Caspar-Bauguil S,Cousin B,Galinier A et al.Adipose tissues as an ancestral immune organ:Site-specific change in obesity.FEBS Lett2005,579:3487–3492.
  • 4Garc??a-Olmo D,Garcia-Arranz M,Herreros D et al.A phase I clinicaltrial of the treatment of Crohn's fistula by adipose mesenchymal stem cell transplantation.Dis Colon Rectum,2005;48:1416 –1423.
  • 5Izadpanah R,Trygg C,Patel B et al.Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue.J Cell Biochem,2006,99:1285–1297.

同被引文献48

  • 1林琴,刘源岗,叶莉,何鹏,王士斌.体外组织构建技术的研究进展[J].生物医学工程研究,2012,31(2):125-129. 被引量：1
  • 2Ju-Yeon Kim,Hong Bae Jeon,Yoon Sun Yang,Wonil Oh,Jong Wook Chang.Application of human umbilical cord blood-derived mesenchymal stem cells in disease models[J].World Journal of Stem Cells,2010,2(2):34-38. 被引量：14
  • 3杨在亮,张波,周继红,黄宏,张良,刘大维.rhBMP-2和rhVEGF转染ADSCs后体外表达及诱导骨缺损成骨修复的实验研究[J].创伤外科杂志,2007,9(4):296-300. 被引量：3
  • 4HanKey D P, McCabe R E, Doberry M J, et al. Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum [ J]. Acta Orthop Scand, 2001, 72 (4) : 395 -403.
  • 5Liu Z J, Ying Z G, Omaida C V. Trafficking and differentiation of mesenchymal stem cells[ J ]. Cell Biochem, 2009, 106 ( 6 ) : 984 - 991.
  • 6Flares - Torales E, Orozco - Barocio A, Gonzalez - Ramella O R, et al. The CD271 expression could be alone for establisher phenotypic marker in bone marrow derivcd mcsenchymal stem cells[J]. Folia Histochem Cytobiol, 2010, 48(4) : 682 - 686.
  • 7Choumerianou D M, Martimianaki G, StIakaki E, et al. Companative study of stemness characteristics of mesenchymal cells from bone rmrrow of children and adults[J]. Cytotherapy, 2010, 12(7): 881 - 887.
  • 8Can A S, Karahuseyinoglu S. Concise review: human umbilical cord stroma with regard to the source of fetus - derived stem cells [J]. Stem Cells, 2007, 25( 11 ) : 2886 - 2895.
  • 9Zhao L, Tang M H, Michael D W, et al. Osteogenic media and rhBMP - 2 induced differentiation of umbilical cord mesenchymal stem cells encapsulated in alginate microbeads and integrated in an injectable calcium phosphate - chitosan fibrous scaffold[J]. Tissue Eng Part A, 2011, 17(7-8) : 969 -979.
  • 10Cao Y, Sun Z, Liao L M, et al. Human adipose tissue - derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo [ J ]. Biochem Biophys Res Commun, 2005, 332(2) : 370 - 379.

引证文献2

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牡丹江医学院学报
2010年 第4期

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