摘要
[目的]建立薏苡非种仁部位中薏苡素的HPLC测定方法。[方法]采用高效液相色谱法。色谱柱为AgilentHC-C18色谱柱(4.6mm×250mm,5μm);流动相为乙腈-0.1%磷酸水(V∶V=25∶75);流速为1.0ml/min;柱温为25℃;检测波长为232nm。[结果]薏苡非种仁部位中薏苡素的含量:根>皮>茎。[结论]该方法准确、专属性强,可为薏苡非种仁部位的综合开发利用提供参考。
[Objective]To establish a method for the determination of coixol in roots,stems and seed capsules of Coix lacryma-jobi L.var.mayuen（Roman.） Stapf herbs by HPLC.[Method]HPLC was used and the conditions were as follows：Agilent HC-C18 column （4.6 mm×250 mm,5 μm） ,mobile phase of acetonitrile-0.1% phosphoric acid （V∶V=25∶75） ,flow rate of 1.0 ml/min,column temperature of 25 ℃ and detection wavelength of 232 nm.[Result]The content of coixol in different parts of Coix lacryma-jobi L.var.ma-yuen（Roman.） Stapf.were as follows：root〉seed capsule〉stem.[Conclusion]This method is accurate and specific,which is useful for the integrative exploitation of in roots,seed capsules and stems of Coix lacryma-jobi L.var.ma-yuen（Roman.） Stapf.
出处
《安徽农业科学》
CAS
北大核心
2010年第28期15586-15587,15589,共3页
Journal of Anhui Agricultural Sciences
基金
贵州省教育厅自然科学类重点科研项目(黔教科2008027)
关键词
薏苡非种仁部位
薏苡素
含量测定
HPLC
Roots
stems and seed capsules of Coix lacryma-jobi L. var. ma-yuen（ Roman. ）Stapf.
Coixol
Content determination
HPLC
