高分子量角蛋白抗体(34βE_(12))在前列腺癌诊断中的应用价值

Use of basal cell specific anti cytokeration antibody (34βE 12 ) as an aid in the diagnosis of prostate cancer

目的：研究高分子量角蛋白抗体（３４βＥ１２）在前列腺癌诊断中的作用。方法：采用微波ＥｎＶｉｓｉｏｎ免疫组织化学方法，观察３４βＥ１２抗体在不同前列腺病变中的染色情况。结果：基底细胞特异性细胞角蛋白抗体３４βＥ１２与所有的前列腺良性病变的基底细胞胞浆特异性染色。１５例前列腺癌中１２例３４βＥ１２染色为阴性，有３例出现阳性反应。原发性前列腺导管癌的癌巢外周有连续或不连续的基底细胞围绕。结论：３４βＥ１２染色阳性不是诊断良性前列腺病变的绝对指标。前列腺癌的诊断必须综合考虑临床、常规组织学检查及免疫组织化学结果才能做出正确的诊断。

Purpose To determine the utility of basal cell specific anti cytokeratin antibody (34βE 12 ) in the diagnosis of prostate cancer. Method Immunoproxidase staining of benign and malignant prostate lesions with 34βE 12 antibody were observed by microwave EnVision immunohistochemical technique. Results The basal cell specific anti cytokeration antibody 34βE 12 stained the basal cells in all of the benign lesions, but 12 of 15 prostate carcinoma lacks immunoreactivity with this antibody, whereas 3 of 15 demonstrate 34βE 12 positive. Prostate transitional cell carcinomas were negative for this antibody. There was a continous or discontiuous layer of basal cells surrounding islands of the primary prostatic duct adenocarcinomas. Conclusion The presence of 34βE 12 siaining should not be considered an absolute indicator of benign prostate lesions. The diagnosis of prostate carcinoma should be based on clinical, routine histologic and immunohistochemical studies.