PDGF-α受体反义寡核苷酸对体外视网膜色素上皮细胞增殖和凋亡的影响

Effect of oligofectamine mediated PDGF-α receptor ASODN transfection on RPE cell proliferation and apoptosis in vitro

目的:探讨血小板源性生长因子-α受体反义寡核苷酸(PDGFR-αASODN)对体外人视网膜色素上皮(humanretinal pigment epithelium,HRPE)细胞增殖和凋亡的作用。方法:使用阳离子脂质体lipofectamineTM2000将靶向PDGFR-α基因的ASODN转染至细胞株HRPE细胞,MTT法检测对HRPE细胞增殖的影响;Hoechst33258荧光染色观察凋亡细胞;流式细胞仪检测HRPE细胞的周期和凋亡率。结果:PDGFR-αASODN转染组细胞增殖抑制率和凋亡率均明显高于对照组(P<0.05)。结论:沉默PDGFR-α基因表达可显著抑制HRPE细胞的增殖,并能诱导其凋亡。

AIM：To investigate the effect of oligofectamine mediated platelet-derived growth factor-α（PDGF-α） receptor antisense oligodeoxyribonucleotide（ASODN） transfection on retinal pigment epithelium（RPE） proliferation and apoptosis in vitro.METHODS：After PDGF-α receptor ASODN was transfected into RPE with lipofectamineTM2000 at different concentrations,the proliferation of RPE was detected by MTT.Hoechst staining was used to display the apoptosis form of RPE.Flow cytometry method was applied to detect the change of cell cycle and apoptosis index.RESULTS：Compared with control group,PDGF-α receptor ASODN group could effectively inhibit the proliferation of RPE;the percentage of G0/G1 phase cells was enhanced and there was significance in increasing apoptosis cells（P0.05）.CONCLUSION：PDGF-α receptor ASODN could induce G0/G1arrest,inhibit cell proliferation,accelerate apoptosis in RPE cells.