大花三色堇和三色堇花粉离体萌发的培养基研究

Study on Culture Medium for in vitro Pollen Germination of Viola×wittrokiana Gams.and Viola tricolor L.

研究了不同浓度的蔗糖和硼酸对大花三色堇和三色堇花粉离体萌发的影响,及大花三色堇不同自交系间花粉萌发的差异。结果表明:蔗糖对花粉离体萌发有着重要的影响,不同蔗糖浓度下,花粉萌发率有明显差异,蔗糖浓度为30%和35%时,大花三色堇和三色堇花粉离体萌发率分别达到最高;添加适量的硼酸有利于离体花粉的萌发,但不同硼酸浓度下,花粉萌发率差异不大;在同样的蔗糖和硼酸浓度下,不同自交系的大花三色堇的花粉萌发率没有明显差异。

Effects of different concentration of sucrose and boric acid on pollen germination in vitro of Viola X wittrockiana Gams,and Viola tricolor L.were studied and the difference of pollen germination of different inbred lines of Pansy.The results showed that sucrose on pollen germination in vitro had an important impact,with different concentrations of sucrose,the pollen germination rates were significantly different,When the sucrose concentration was 30%,the pollen germination rate in vitro of Viola×wittrockiana Gams.was highest,When the sucrose concentration was 35%,the pollen germination rate in vitro of Viola tricolor L.was highest;Adding the appropriate amount of boric acid was beneficial to the pollen germination in vitro,but with different concentrations of boric acid,there was little different in pollen germination rate;In the same germination condition,there were no significant difference in the pollen germination rate with different inbred lines of Pansy.