睡眠剥夺对大鼠学习记忆、海马IL-1β表达的影响及SB203580干预作用

Effect of Sleep Deprivation on Learning-memory and Hippocampal IL-1β in Rats and the Role of SB203580 Intervention

目的睡眠剥夺(SD)对大鼠学习记忆、海马IL-1β表达的影响及SB203580干预作用。方法 90只雄性Sprague-Dawley大鼠随机分成对照组、模型组、SB203580干预组。改良多平台法建立SD模型。光镜观察海马区神经细胞形态变化,免疫组化法和Western blot法检测海马区IL-1β蛋白的表达,水迷宫法测试动物学习记忆功能。结果与对照组比较,模型组中,随睡眠剥夺时间延长,SD大鼠海马神经元结构损伤明显,存活神经元密度降低、IL-1β阳性细胞数及表达增多,动物学习记忆能力减退;与模型组比较,SB203580组中脑组织形态损伤程度减轻、存活神经元密度增加、IL-1β表达回降,动物学习记忆功能得到改善。结论 SD可增加海马IL-1β表达,引起动物认知功能障碍,SB203580可能通过降低海马IL-1β表达,改善SD大鼠学习记忆功能。

Objective To investigate the effects of sleep deprivation （SD） on learning-memory and hippocampal IL-1β in Rats and the role of SB203580 intervention.Methods Male Sprague- Dawley rats were randomly divided into three groups： control,model,SB203580-treated groups.SD models were established by the modified multiple platform methods.Morphological changes were observed with light microscopy.The IL-1β expression was examined by immunohistochemistry and Western blot.Learning and memory function was monitored with Morris water maze.Results Compared with control group,neuronal structure was obviously damaged,the density of survival neurons decreased,the IL-1β expression increased and learning and memory function significantly decreased with time of SD in model group.Compared with model group,the density of survival neurons was increased,IL-1β expression obviously decreased,but the ability of learning and memory function enhanced in SB203580-treated group （P 〈 0.05）.Conclusions SD could increase IL-1β expression and finally result in cognition injury.However,SB203580 improved learning-memory function by attenuating IL-1β expression.