CPO在金-碳纳米管修饰电极上的固定化及应用

The immobilization of chloroperoxidase on gold and carbon nanotubes modified electrode with enhanced catalytic activity

应用电化学方法在碳纳米管修饰的玻碳电极上沉积金,继而固定氯过氧化物酶(CPO),制得的CPO-Au/SWNTs/GC修饰电极的循环伏安曲线上呈现一对对称的氧化还原电流峰,说明CPO在金-碳纳米管复合修饰膜上可进行直接的电子传递,并且是一个受吸附控制的准可逆电极过程.循环伏安行为与溶液的pH值密切相关,是典型的一电子一质子反应.修饰电极性能稳定,对氧的电化学还原具有很好的催化作用,可应用于原位产生过氧化氢下CPO催化的有机合成反应.

The gold nano-particles were electrochemically deposited onto the single wall carbon nanotubes（SWNTs） modified glassy carbon electrode（GCE）,then the Chloroperoxidase（CPO） was immobilized on it.A pair of well-defined reduction and oxidation peaks in the cyclic voltammogram were observed at the CPO-Au/SWNTs/GC modified electrode,indicating that the direct electron transfer reaction between CPO and Au-SWNTs composite modified electrode occurred and followed by a quasi-reversible process controlled by surface adsorption.The results of cyclic voltammetry showed that the electrode reaction of CPO displayed a good relationship with pH of the solution,corresponding to a process of one electron and one proton reaction.The modified electrode showed a good stanbility and excellent catalytic activity for electrochemical reduction of O 2 could be used for catalyse organic synthesis reaction with on-line generated hydrogen peroxide