HPLC测定经络贴巴布剂中3种皂苷的含量

Determination of three ginsenosides in Jingluo cataplasm by HPLC

目的建立经络贴巴布剂中三种皂苷成分的含量测定方法。方法采用高效液相色谱法,色谱柱为kromasil-C18(250 mm×4.6 mm,5μm)柱,乙腈-水(梯度洗脱)为流动相,检测波长203 nm,流速1 mL.min-1,柱温40℃。结果人参皂苷Rg1在0.82～8.2μg范围内与峰面积具有良好的线性关系(r=0.999 6),平均回收率为98.33%,RSD=2.723%(n=6);人参皂苷Rb1在0.3256～3.256μg范围内与峰面积具有良好的线性关系(r=0.999 9),平均回收率为99.30%,RSD=0.918%(n=6);三七皂苷R1在0.201～2.01μg范围内与峰面积具有良好的线性关系(r=0.999 8),平均回收率为97.90%,RSD=0.695%(n=6)。结论本方法准确、灵敏、重现性好,阴性无干扰,可用于经络贴中3种皂苷类成分的质量控制。

Objective To establish a HPLC method for the determination of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in Jingluo cataplasm.Methods Kromasil-C18 column（250 mm×4.6 mm,5 μm） was used at 40 ℃,with the mobile phase consisted of acetonitrile-water（gradient elution） at a flow rate of 1 mL·min-1,and 203 nm as detected wavelength.Results The ginsenoside Rg1 showed good linearity in the range of 0.82-8.2 μg（r=0.999 6） and the average recovery was 98.33%（RSD=2.723%）.The ginsenoside Rb1 showed good linearity in the range of 0.325 6-3.256 μg（r=0.999 9） and the average recovery was 99.30%（RSD=0.918%）.The notoginsenoside R1 showed good linearity in the range of 0.201-2.01 μg（r=0.999 8） and the average recovery was 97.90%（RSD=0.695%）.Conclusion The method is accurate,sensitive,reproducible and is suitable for the quality control of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in Jingluo cataplasm.