云南省玉米大斑病菌生理小种的鉴定及交配型分析

Identification of physiological races and analysis of mating type of Setosphaeria turcica in Yunnan Province

采用2套Ht单基因鉴别品种,对2007-2009年采自云南省13个地区的81份玉米大斑病菌菌株进行了生理小种鉴定,利用分子标记技术对菌株交配型进行了分析。结果表明,云南省玉米大斑病菌生理小种和交配型组成复杂,81个菌株可分为0、1、2、3、12、23、2N、3N、12N、23N和123N等11个小种类型,其中能克服鉴别寄主上所有显性抗病基因的123N小种占22.2%,为优势小种;依据分子标记,81个菌株可分成A、a、Aa 3种交配型菌株及中性菌株,分别占49.4%、12.3%、29.6%和8.6%。此外,在同一区域内存在多个生理小种和不同的交配型菌株。

Eighty one Setosphaeria turcica isolates were collected from 13 corn planting areas of Yunnan Province during 2007 to 2009,which were identified by using two sets of the corn differentials containing single Ht resistance genes and their mating types were analyzed by using molecular markers.The results showed that both physiological races and mating types of the pathogen were very complicated in Yunnan Province.The isolates could be divided into 11 races including 0,1,2,3,12,23,2N,3N,12N,23N and 123N,among which 123N could overcome all of the single dominant resistance genes of the differentials to S.turcica and accounted for 22.2%.Based on the molecular markers,the 81 isolates were divided into three mating types including A,a and Aa,and neuter,accounting for 49.4%,12.3%,29.6% and 8.6%,respectively.In addition,different races and mating types of S.turcica existed in the same area.