同种异体血小板诱导PVR动物模型研究

RESEARCH ON THE PVR MODEL INDUCED BY HOMOLOGOUS PLATELET

目的：用同种异体富含血小板血浆（platelet－richplasms，PRP）诱导增殖性玻璃体视网膜病变（prolifera－tivevitreoretinopathy，PVR）动物模型，探讨其制模机理及与人PVR病理相似性。方法：取同种异体富含血小板血浆注射入兔眼内，直接检眼镜动态检查、病理切片观察。结果：PRP复制PVR动物模型方法成功率达75％；动态观察和病理切片显示注射PRP后眼内有炎症反应、细胞增殖及膜的形成，造成视网膜脱离合并PVRB级。结论：该方法成功率较高，方法简便易行，模型基本模拟人PVR的病理过程，适合科研和实验动物学教学使用。

Objective: To study the mechanism of PVR model induced by homologous platelet-rich plasms(PRP) and the sumilarity in comparison with the PVR on hclman beings. Methods: Rabbit reaction was followed up ophthalmoscopically and histopathologically after vitrous injection of homologous PRP was administered.Results:The positive of PVR model induced by homologous platelet-rich plasms reached 75% and the retinal detachment accompanied grade B of PVR, the inflammation, proliferous cell and membrane were observed ophthalmoscopically and histopathologically.Conclusions There were a high positive and an easy operation from PVR model induced by homologous platelet - rich plasms. The histopathology of model was similar to PVR on human. The model was available to scientific research and experiment.