HPLC法测定益心酮片中五种成分的含量

Determination of Five Components in Yixintong Tablets by HPLC

目的：建立HPLC同时测定益心酮片中牡荆素-4＂-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法：采用HPLC法，色谱柱为Sepax HP C18柱（4．6 mm×250mm，5μm）；流动相：A为乙腈-四氢呋喃（20：1），B为0．5％甲酸溶液，采用梯度洗脱；0～10min，A为14％～17％，B为86％～83％；10—26min，A为17％～18％，B为83％～82％；26～28min，A为18％-14％．B为82％～86％；检测波长360nm，流速1．0mL·min；柱温32℃。结果：该方法加样回收率牡荆素-4＂-O-葡萄糖苷为99．61％（RSD=0．64％），牡荆素鼠李糖苷为99．30％（RSD=0．49％），牡荆素为98．97％（RSD=1．03％），芦丁为99．45％（RSD=0．85％）．金丝桃苷为99．27％（RSD=0．96％）。结论：该方法准确可靠，重现性好，可用于益心酮片的质量控制。

Objective：To establish an HPLC method for determining simultaneously vitexin 4＂-O-glucoside, vitexin rhamnoside, vitexin, rutin and hyperoside in Chinese patent medicine Yixintong Tablets. Methods：The HPLC method was carried out on Sepax HP C18 column （4. 6 mm×250 mm, 5μm） at the temperature 32℃ ;the mobile phase consisted of A：CH3CN-THF（20：1 ）, B：0. 5% Formic Acid gradient elution profile was 0 - 10 min, A ： 14% - 17% , B ： 86% - 83% ; 10 - 26 min, A ： 17% - 18%, B ： 83% - 82% ;26 - 28 min, A：18% - 14% B ：82% -86% ;UV detection wavelength was set at 360 nm. The flow rate was 1.0 mL · min-1. Results：The average recoveries were 99. 61% with RSD 0. 64% for vitexin 4＂-O-glucoside ;99.30% with RSD 0. 49% for vitexin rhamnoside ;98.97% with RSD 1.03% for vitexin;99.45% with RSD 0. 85% for rutin;99.27% with RSD 0. 96% for hyperoside. Conclusions：The method is simple and it has the adequate accuracy and selectivity. It can be used to control quality of Yixintong Tablets.